Issue 1, 2024

Visualization of peroxynitrite/GSH cross-talk in the oxidant–antioxidant balance by a dual-fluorophore and dual-site based mito-specific fluorescent probe

Abstract

Peroxynitrite (ONOO) and glutathione (GSH) play mutually regulating roles in the oxidant–antioxidant balance of organisms, which has a profound relationship with people's health and disease. In this study, we designed a two-photon fluorescent probe CD-NA that could simultaneously detect ONOO and GSH via dual-fluorophore and dual-site properties. CD-NA shows different fluorescence responses to ONOO (annihilated red fluorescence) and GSH (enhanced green emission) with high specificity and sensitivity. Notably, the response of CD-NA to ONOO was unaffected by GSH, and the reverse is also true. It allows the ONOO/GSH cross-talk to be successfully imaged. Given these excellent properties, CD-NA has been favorably employed in detecting ONOO and GSH in living cells with the ability to target mitochondria. Therefore, CD-NA offers an efficient method for understanding the oxidant–antioxidant balance and interrelated physiological functions of ONOO and GSH in living systems, and provides a new strategy to sort out the complex relationships and roles of various analytes in complex physiological processes.

Graphical abstract: Visualization of peroxynitrite/GSH cross-talk in the oxidant–antioxidant balance by a dual-fluorophore and dual-site based mito-specific fluorescent probe

Supplementary files

Article information

Article type
Paper
Submitted
01 Jun 2023
Accepted
20 Nov 2023
First published
24 Nov 2023

Org. Biomol. Chem., 2024,22, 159-168

Visualization of peroxynitrite/GSH cross-talk in the oxidant–antioxidant balance by a dual-fluorophore and dual-site based mito-specific fluorescent probe

Y. Liu, J. Zhao and Y. Wang, Org. Biomol. Chem., 2024, 22, 159 DOI: 10.1039/D3OB00872J

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