Polyketides with α-glucosidase inhibitory and neuroprotective activities from Aspergillus versicolor associated with Pedicularis sylvatica

Abstract

Aspergillus versicolor, an endophytic fungus associated with the herbal medicine Pedicularis sylvatica, produced four new polyketides, aspeversins A–D (1–2 and 5–6) and four known compounds, O-methylaverufin (2), aversin (3), varilactone A (7) and spirosorbicillinol A (8). Their structures were elucidated by extensive spectroscopic data analysis, and their absolute configurations were determined by calculated electronic circular dichroism (ECD) and Mo₂(AcO)₄-induced CD data. Compound 5 was found to exhibit α-glucosidase inhibitory activity with an IC₅₀ value of 25.57 μM. An enzyme kinetic study indicated that 5 was a typical uncompetitive inhibitor toward α-glucosidase, which was supported by a molecular docking study. Moreover, compounds 1–3 and 5 also improved the cell viability of PC12 cells on a 1-methyl-4-phenylpyridinium (MPP⁺)-induced Parkinson's disease model, indicating their neuroprotective potential as antiparkinsonian agents.