Ultrasound-assisted magnetic dispersive micro-solid-phase extraction based on carbon quantum dots/zeolite imidazolate framework-90/polyvinyl pyrrolidone/Fe3O4 followed by high-performance liquid chromatography with ultraviolet detection for trace analysis of paracetamol and etodolac in human plasma

Abstract

A four-part and sustainable nanocomposite composed of carbon quantum dots modified with zeolite imidazolate framework-90, polyvinyl pyrrolidone and magnetite (CQDs/ZIF-90/PVP/Fe₃O₄) was fabricated and applied in ultrasound-assisted magnetic dispersive micro-solid-phase extraction (US-A-MDMSPE). US-A-MDMSPE was followed by high-performance liquid chromatography with ultraviolet detection (HPLC-UV) for extraction, enrichment and simultaneous low-level monitoring of paracetamol (PCM) and etodolac (EDL) in human plasma. To increase the extraction yield and improve the sensitivity, nanohybrid arrays of metal–organic frameworks and conductive polymers were immobilized on the surface of CQDs followed by magnetization. The nano-extractor was characterized via Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), field-emission scanning electron microscopy (FE-SEM) and energy-dispersive X-ray spectroscopy (EDX). The recent method provided limits of detection (LODs) of 0.16 and 0.09 ng mL⁻¹ for PCM and EDL, respectively. The calibration curve for PCM and EDL was linear in the range of 0.7–2000 ng mL⁻¹ and 0.5–1200 ng mL⁻¹ with a regression (r²) value between 0.993 and 0.998, respectively. Acceptable precisions including intra-assay (≤6.9%) and inter-assay (≤8.3%) accuracies and notable accuracy (≤8.5%) were achieved to demonstrate the applicability of this method for the evaluation of the pharmacokinetic data of target drugs in human plasma.