Issue 41, 2024

A fluorescent ratiometric potassium sensor based on IPG4-silica microparticles for selective detection and fluorescence imaging of potassium cations

Abstract

Potassium cations play many important roles in living organisms, especially in electro-physiology, since they are involved in neurotransmission and muscle contractions. We report the synthesis of a ratiometric fluorescent microsensor for potassium (K+) detection, based on the fluorescent probe ION potassium green 4. Potassium-sensitive fluorescent microparticles were obtained by using silica as the core material. We obtained silica-based microsensors with sizes in the micrometer range, spherical shapes, good monodispersity, optimal selectivity and a sensitivity range of 0 to 40 mM. The microsensors also proved to be non-toxic in cell cultures and suitable for fluorescence imaging, offering new possibilities for non-invasive optical detection, quantification and in situ monitoring of K+ variations in cell culture systems.

Graphical abstract: A fluorescent ratiometric potassium sensor based on IPG4-silica microparticles for selective detection and fluorescence imaging of potassium cations

Supplementary files

Article information

Article type
Paper
Submitted
14 May 2024
Accepted
19 Aug 2024
First published
27 Aug 2024
This article is Open Access
Creative Commons BY-NC license

J. Mater. Chem. B, 2024,12, 10573-10583

A fluorescent ratiometric potassium sensor based on IPG4-silica microparticles for selective detection and fluorescence imaging of potassium cations

F. Colella, S. Forciniti, V. Onesto, G. Grasso, H. Iuele, G. Gigli and L. L. del Mercato, J. Mater. Chem. B, 2024, 12, 10573 DOI: 10.1039/D4TB01047G

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