Enhanced wound healing with biogenic zinc oxide nanoparticle-incorporated carboxymethyl cellulose/polyvinylpyrrolidone nanocomposite hydrogels

Abstract

Contemporary wound dressings lack antibacterial properties, exhibit a low water vapour transmission rate, and demonstrate inadequate porosity. In order to overcome these limitations, scientists have employed water hyacinth to produce carboxymethyl cellulose (CMC). CMC/PVP nanocomposite films containing biogenic zinc oxide nanoparticles (nZnOs) were synthesised using cost effective solution-casting technique. As the proportion of nZnOs in the film increased, swelling and water permeability decreased, whereas mechanical stability improved. Dynamic light scattering testing and transmission electron microscopy confirmed that the particle size was around 50.7 nm. Field emission scanning electron microscopy (FESEM) images showed that nZnOs were distributed uniformly in the polymer matrix. Cell viability against Vero cells was greater than 94%, and a substantial zone of inhibition against S. aureus and E. coli bacteria was observed. Wounds of albino mice were treated with CMC/PVP and CMC/PVP/nZnO (6%) nanocomposite hydrogels and healed in 20 and 12 days, respectively, as demonstrated by wound healing assay and histological staining. In vitro and in vivo studies revealed that the novel nanocomposite hydrogels exhibit improved cell viability and wound healing features. Therefore, they could be exploited as promising skin wound dressing materials.