Biocompatible hydrogel coating on single living cells through visible light-induced polymerization

Abstract

Visible light-mediated photocatalysis leads to the efficient hydrogel coating of individual mammalian cells, functionalized with biocompatible anchor molecules tagged with fluorescein serving as a trifecta: photocatalyst, initiator, and fluorophore. NIH3T3 fibroblast cells are encapsulated within hydrogel shells of poly(ethylene glycol) diacrylate (PEGDA) and N-vinylpyrrolidone without any noticeable decrease in cell viability.