Ligand binding characteristics of an NAD+ riboswitch revealed by FRET and biolayer interferometry

Nico E. Conoan Nieves; Julia R. Widom

doi:10.1039/D4CC04261A

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Ligand binding characteristics of an NAD⁺ riboswitch revealed by FRET and biolayer interferometry†

Nico E. Conoan Nieves

^a and Julia R. Widom

*^a

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* Corresponding authors

^a University of Oregon Department of Chemistry and Biochemistry, Eugene, USA
E-mail: jwidom@uoregon.edu

Abstract

The Class II NAD⁺ riboswitch is a bacterial RNA that binds ligands containing nicotinamide. Herein, we report a fluorescence and biolayer interferometry study of riboswitch interactions with β-NMN. The results reveal a shift in the prevalence of a pseudoknot structure in the presence of ligand and Mg²⁺.

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Article information

DOI: https://doi.org/10.1039/D4CC04261A
Article type: Communication
Submitted: 21 Aug 2024
Accepted: 18 Nov 2024
First published: 19 Nov 2024
This article is Open Access

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Chem. Commun., 2025,61, 346-349

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Ligand binding characteristics of an NAD⁺ riboswitch revealed by FRET and biolayer interferometry

N. E. Conoan Nieves and J. R. Widom, Chem. Commun., 2025, 61, 346 DOI: 10.1039/D4CC04261A

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