Classification of self-assembled fluorescent probes and their application in cancer diagnosis

Abstract

The high sensitivity, high selectivity, real-time monitoring capability, non-destructiveness, and versatility of small molecule fluorescent probes make them indispensable and powerful tools in bioscience research. Self-assembling fluorescent probes are a novel type of material that spontaneously assemble fluorescent dyes with specific molecules into nanoscale structures. Compared with ordinary small molecule fluorescent probes, self-assembled fluorescent probes have higher stability, selectivity, sensitivity, and temporal stability in detection. In recent years, the incidence and mortality of cancer have increased year by year, which has brought great challenges to the safety of human life, and traditional diagnostic methods such as nuclear magnetic resonance, ultrasound diagnosis, and X-ray tomography are time-consuming and have low resolution. The boundary between normal tissue and cancer tissue cannot be accurately distinguished during surgical resection, resulting in the possibility of recurrence after surgery. Fluorescent probes can quickly and efficiently diagnose and label cancerous tumor cells, which is of great significance for cancer discovery and treatment. In this paper, we review the classification of self-assembled fluorescent probes (molecular self-assembled fluorescent probes, nanomaterial self-assembled fluorescent probes and biological macromolecule self-assembled fluorescent probes) that are used in identifying and imaging cancerous tumor tissues. Furthermore, we discuss the current problems faced by self-assembled fluorescent probes through the specific identification and monitoring of enzymes with abnormal contents, active substances and low pH in the tumor microenvironment, hoping to give readers more inspiration.