Issue 26, 2025
From the journal:

Chemical Communications

Construction of a dual-color fluorescent light-up biosensor based on sequential coding for label-free and sensitive detection of multiple Piwi-interacting RNAs†

Jinping Hu,‡a   Jie Zhang,‡a   Juan Hu ORCID logo *a  and  Chun-yang Zhang ORCID logo *a  

* Corresponding authors

a School of Chemistry and Chemical Engineering, State Key Laboratory of Digital Medical Engineering, Southeast University, Nanjing 211189, China
E-mail: hujuan@seu.edu.cn, zhangcy@seu.edu.cn

Abstract

We construct a dual-color light-up biosensor for label-free and sensitive detection of multiple piRNAs. This biosensor exhibits high sensitivity with a limit of detection of 3.19 aM for piRNA-36026 and 4.71 aM for piRNA-823. Moreover, it can accurately measure cellular piRNA levels with single-cell sensitivity, and discriminate breast cancer tissues from healthy tissues.

Graphical abstract: Construction of a dual-color fluorescent light-up biosensor based on sequential coding for label-free and sensitive detection of multiple Piwi-interacting RNAs

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Article information

DOI
https://doi.org/10.1039/D4CC06421F
Article type
Communication
Submitted
05 Dec 2024
Accepted
05 Mar 2025
First published
05 Mar 2025
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2025,61, 5031-5034

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Construction of a dual-color fluorescent light-up biosensor based on sequential coding for label-free and sensitive detection of multiple Piwi-interacting RNAs

J. Hu, J. Zhang, J. Hu and C. Zhang, Chem. Commun., 2025, 61, 5031 DOI: 10.1039/D4CC06421F

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