Flow cell for high throughput Raman spectroscopy of non-transparent solutions

Abstract

This work introduces a high-throughput setup for Raman analysis of various flowing fluids, both transparent and non-transparent. The setup employs a microfluidic cell, used with an external optical setup, to control the sample flow's position and dimensions via 3-dimensional hydrodynamic focusing. This approach, in contrast to the prevalent use of fused silica capillaries, reduces the risk of sample photodegradation and boosts measurement efficiency, enhancing overall system throughput. The microfluidic cell has been further evolved to laminate two distinct flows from different samples in parallel. Using line excitation, both samples can be simultaneously excited without moving parts, further increasing throughput. This setup also enables real-time monitoring of phenomena like mixing or potential reactions between the two fluids. This development could significantly advance the creation of highly sensitive, high-throughput sensors for fluid composition analysis.