Development and characterization of a Dextran/CaCl2-based blood-mimicking fluid: a comparative study of rheological and mechanical properties in artificial erythrocyte suspensions

Abstract

The development of accurate blood-mimicking fluids (BMFs) is essential for in vitro studies of blood contacting medical devices. Experimental data typically relies on single-phase glycerin/water solutions as substitutes to visualize simplified blood flow. These models are accurate only at high shear rates, limiting their applicability at lower shear rates. In this study, we investigated three potential BMFs, each composed of poly(sodium acrylate-co-acrylamide) hydrogel microparticles (beads) as artificial erythrocytes. Microbeads were produced using microfluidic systems (MFS) and were suspended in three plasma-like solutions: 10% and 50% (v/v) glycerol/water solutions and a Dextran40/CaCl₂ solution. The BMFs were evaluated for their rheological and mechanical properties, including particle elasticity, sedimentation behavior, and shear flow analysis, to assess their suitability for mimicking blood. Rheometric measurements were performed at room temperature using a plate-plate configuration, measuring viscosity and shear stress for shear rates of 5–500 s⁻¹. Atomic force microscopy (AFM) measurements were conducted to assess their mechanical response. The Dextran40/CaCl₂-based BMF was identified as the most promising, demonstrating rheological and mechanical properties that closely align with those of human blood. This research offers a refined approach to developing blood analogs that better simulate the mechanical response and flow characteristics of blood for the validation and development of blood contacting medical devices.