Issue 13, 2012
From the journal:

Analyst

Using a nanopore for single molecule detection and single cell transfection†

Edward M. Nelson,a   Volker Kurz,a   Jiwook Shim,a   Winston Timp*b  and  Gregory Timp*a  

* Corresponding authors

a Stinson-Remick Hall, University of Notre Dame, Notre Dame, IN 46556, USA
E-mail: gtimp@nd.edu
Tel: +1-574-631-1272

b Johns Hopkins University, 855 N. Wolfe St., Rangos 580.19, Baltimore, MD, USA
E-mail: wtimp1@jhmi.edu
Tel: +1-617-642-9402

Abstract

We assert that it is possible to trap and identify proteins, and even (conceivably) manipulate proteins secreted from a single cell (i.e. the secretome) through transfection via electroporation by exploiting the exquisite control over the electrostatic potential available in a nanopore. These capabilities may be leveraged for single cell analysis and transfection with single molecule resolution, ultimately enabling a careful scrutiny of tissue heterogeneity.

Graphical abstract: Using a nanopore for single molecule detection and single cell transfection

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Article information

DOI
https://doi.org/10.1039/C2AN35571J
Article type
Paper
Submitted
13 Feb 2012
Accepted
22 May 2012
First published
23 May 2012

Analyst, 2012,137, 3020-3027

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Using a nanopore for single molecule detection and single cell transfection

E. M. Nelson, V. Kurz, J. Shim, W. Timp and G. Timp, Analyst, 2012, 137, 3020 DOI: 10.1039/C2AN35571J

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