Issue 91, 2012

Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

Abstract

Two new dibenzocyclooctyne–thymidine monomers were incorporated into oligonucleotides and crosslinked to azide-labelled complementary strands across the DNA grooves. Equivalent reactions were successful using a (bicyclo[6.1.0]nonyne) alkyne. Oligonucleotides containing internal cyclooctyne and amino groups were simultaneously reacted with azides and NHS esters of different fluorescent dyes to produce functional genetic probes.

Graphical abstract: Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

Supplementary files

Additions and corrections

Article information

Article type
Communication
Submitted
16 Jul 2012
Accepted
01 Aug 2012
First published
03 Aug 2012

Chem. Commun., 2012,48, 11184-11186

Fast and efficient DNA crosslinking and multiple orthogonal labelling by copper-free click chemistry

M. Shelbourne, T. Brown, A. H. El-Sagheer and T. Brown, Chem. Commun., 2012, 48, 11184 DOI: 10.1039/C2CC35084J

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