Probing subcellular organic hydroperoxide formation via a genetically encoded ratiometric and reversible fluorescent indicator

Boxuan Simen Zhao; Gong Zhang; Shizhe Zeng; Chuan He; Peng R. Chen

doi:10.1039/C3IB40209F

Probing subcellular organic hydroperoxide formation via a genetically encoded ratiometric and reversible fluorescent indicator†

Boxuan Simen Zhao,^ab Gong Zhang,^ac Shizhe Zeng,^d Chuan He^ab and Peng R. Chen*^ac

* Corresponding authors

^a Synthetic and Functional Biomolecules Center (SFBC), Beijing National Laboratory for Molecular Sciences (BNLMS), Department of Chemical Biology, College of Chemistry, Peking University, Beijing, China
E-mail: pengchen@pku.edu.cn
Tel: +86 10-62755773

^b Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, USA

^c Peking-Tsinghua Center for Life Sciences, Beijing, China

^d International Curriculum Center, High school affiliated to Renmin University, Beijing, China

Abstract

A ratiometric and reversible organic hydroperoxide (OHP) sensor, rOHSer, was developed with high sensitivity and selectivity for subcellular OHP visualization. Through targeting rOHSer to the nucleus, we demonstrated that high levels of D-glucose cause elevated OHP production in this compartment. Further utilization of rOHSer probe may allow more elucidation of unique roles of OHPs in diverse biological processes.

This article is part of the themed collection: Synthetic Biology 2012-2014

Integrative Biology

Probing subcellular organic hydroperoxide formation via a genetically encoded ratiometric and reversible fluorescent indicator†

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