Sensitive, highly resolved, and quantitative 1H–13C NMR data in one go for tracking metabolites in vegetal extracts

Caroline Mauve; Soumaya Khlifi; Françoise Gilard; Grégory Mouille; Jonathan Farjon

doi:10.1039/C6CC01783E

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Sensitive, highly resolved, and quantitative ¹H–¹³C NMR data in one go for tracking metabolites in vegetal extracts†

Caroline Mauve,^ab Soumaya Khlifi,^abc Françoise Gilard,^ab Grégory Mouille^d and Jonathan Farjon*^c

Author affiliations

* Corresponding authors

^a Plateforme Métabolisme-Métabolome, Institute of Plant Sciences Paris Saclay IPS2, CNRS, INRA, Université Paris-Sud, Université Evry, Université Paris-Saclay, Paris Diderot, Sorbonne Paris-Cité, bât 630, 91405 Orsay, France

^b Plateforme Métabolisme-Métabolome, Institute of Plant Sciences Paris-Saclay IPS2, Paris Diderot, Sorbonne Paris-Cité, bât 630, 91405 Orsay, France

^c Université Paris – Sud, équipe de RMN en milieu orienté, Institut de Chimie Moléculaire et des Matériaux d'Orsay (ICMMO), Université Paris-Saclay, UMR CNRS 8182, bât. 410, Rue du doyen Georges Poitou, 91405 Orsay Cedex, France
E-mail: jonathan.farjon@u-psud.fr

^d INRA centre Versailles-Grignon, Institut Jean Pierre Bourgin, UMR 1318 INRA-AgroParis Tech, bât 2, route de saint-Cyr, 78026 Versailles cedex, France

Abstract

The quantification of metabolites is essential for understanding and improving biological systems. With the aim to quantify in one map a complex mixture composed of low concentrated metabolites, a new experiment called the ¹H–¹³C QUIPU HSQC allows improving of both resolution and sensitivity for investigation of vegetal extracts.

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Article information

DOI: https://doi.org/10.1039/C6CC01783E
Article type: Communication
Submitted: 01 Mar 2016
Accepted: 30 Mar 2016
First published: 13 Apr 2016

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Chem. Commun., 2016,52, 6142-6145

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Sensitive, highly resolved, and quantitative ¹H–¹³C NMR data in one go for tracking metabolites in vegetal extracts

C. Mauve, S. Khlifi, F. Gilard, G. Mouille and J. Farjon, Chem. Commun., 2016, 52, 6142 DOI: 10.1039/C6CC01783E

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Chemical Communications