A perfluoroaromatic abiotic analog of H2 relaxin enabled by rapid flow-based peptide synthesis

Abstract

H2 relaxin is a pleiotropic peptide hormone with clinical potential. Here we report on the reaction and use of hexafluorobenzene as an intramolecular disulfide replacement between Cys10 and Cys15 in the A-chain of H2 relaxin. Using flow-based Fmoc solid-phase peptide synthesis methodology we were able to obtain high-quality H2 relaxin fragments that were previously reported as challenging to synthesize. Subsequent native chemical ligation and oxidative folding enabled total synthesis of both wild type H2 relaxin and a C₆F₄ linked analog. Cell-based activity assays revealed modest activity for the C₆F₄ linked H2 relaxin analog, albeit 100-fold reduced relative to wild type. This work demonstrates how perfluoroarylation-cysteine S_NAr chemistry may be a useful tool for the selective replacement of native disulfide bonds in proteins.