Issue 91, 2017

A fast-response fluorescent probe for hypochlorous acid detection and its application in exogenous and endogenous HOCl imaging of living cells

Abstract

A facile fluorescent probe (NBD-DOP) has been developed to detect hypochlorous acid (HOCl) in this study. The probe consists of a NBD fluorophore and a dopamine moiety that reacts with HOCl specifically. The dopamine group quenches the fluorescence of NBD efficiently through a photoinduced electron transfer (PET) effect. Experimental data showed that NBD-DOP could detect HOCl with ultrafast response, high sensitivity and high selectivity in a wide pH range. The probe could also be used to detect the myeloperoxidase enzyme that produces HOCl. Moreover, NBD-DOP has been applied in the imaging of exogenous and endogenous HOCl in living cells by confocal fluorescence microscopy.

Graphical abstract: A fast-response fluorescent probe for hypochlorous acid detection and its application in exogenous and endogenous HOCl imaging of living cells

Supplementary files

Article information

Article type
Communication
Submitted
21 Sep 2017
Accepted
26 Oct 2017
First published
26 Oct 2017

Chem. Commun., 2017,53, 12349-12352

A fast-response fluorescent probe for hypochlorous acid detection and its application in exogenous and endogenous HOCl imaging of living cells

Y. Jiang, G. Zheng, N. Cai, H. Zhang, Y. Tan, M. Huang, Y. He, J. He and H. Sun, Chem. Commun., 2017, 53, 12349 DOI: 10.1039/C7CC07373A

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