Natália Vilaçaa,
Ana F. Machadoa,
Filipa Morais-Santosbc,
Ricardo Amorimbc,
A. Patrícia Netoa,
Enora Logodina,
Manuel F. R. Pereirad,
Mariana Sardoe,
João Rochae,
Pier Parpota,
António M. Fonsecaa,
Fátima Baltazar*bc and
Isabel C. Neves*a
aCentre of Chemistry, Chemistry Department, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. E-mail: ineves@quimica.uminho.pt
bLife and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus Gualtar, Braga, Portugal. E-mail: fbaltazar@med.uminho.pt
cICVS/3B's – PT Government Associate Laboratory, Guimarães, Braga, Portugal
dLaboratory of Catalysis and Materials (LCM), Associate Laboratory LSRE-LCM, Faculdade de Engenharia, University of Porto, Rua Dr Roberto Frias, 4200-465 Porto, Portugal
eCICECO – Chemistry Department, University of Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal
First published on 24th February 2017
Several silica microporous structures have been studied for their potential as drug delivery systems (DDS) over the last years. However, systematic studies comparing host structures with different topologies and particle sizes, and toxicity studies to human cancer cells, are scarce. In the present work, 3D crystalline structures, three different zeolites (large, medium and small pore size) and one titanosilicate (large pore size) were used as hosts for loading 5-fluorouracil (5-FU), an anticancer drug currently used to treat several malignant tumors. Here, we (i) compared the loading capacity and drug release profiles of the different hosts in simulated body fluid conditions, including host structure stability studies; (ii) established the kinetic parameters for the release of 5-FU and (iii) studied the effect of 5-FU encapsulation in the viability of human breast and colon cancer cells, with determination of the potentiation factor. The loading capacity and the release profile of the DDS were revealed to be dependent on the porous framework of the host structures. Decrease in pH to 2.0 (simulation of gastro-intestinal fluid), showed stability of the host structures, with minimal leaching of Al3+ and no Ti4+ for long periods of time (up to 72 h). All DDS drug release profiles fitted the Weibull model. These silica microporous structures were revealed to be non-toxic to the cancer cells, while all DDS endorsed the important 5-FU potentiation effect on cell viability.
An ideal DDS is a system that should improve drug bioavailability, prevent its premature degradation, also potentiating its effect. Importantly, the systems must maintain drug concentrations within their therapeutic window, by controlling the drug release rates, and target the pharmacological site at effective concentrations, but with low cytotoxicity.1,2
In this context, inorganic nanomaterials have a range of structural and physical properties that are suitable for therapeutic delivery systems. The sizes, topologies, and surfaces of inorganic nanomaterials can be tailored to produce distinct interactions with both in vitro and in vivo biological systems.3 In this class of materials, silica-based structures, such as microporous crystalline titanosilicates and zeolites, mesoporous silica and amorphous or/and crystalline silica particles, are important materials with a wide range of applications.
Thus, zeolites and titanosilicate materials are porous nanomaterials with many potential applications in the biomedical field. There are several examples of zeolite application reported in the literature, including for drug delivery4–6 and imaging.7–9 These examples show that different zeolite structures can be exchanged with cations, functionalized or loaded with drug molecules for specific biomedical applications.10 In the case of the microporous titanosilicate materials, recent studies show that these materials have potential as drug carriers, with low cytotoxicity.11–13
The goal of this work is to study the potential of several silica microporous structures as hosts for 5-fluorouracil (5-FU) as drug delivery systems for in vitro models of colorectal and breast cancers. 5-FU is a pyrimidine analog antimetabolite, with a general spectrum of activity against solid tumors, such as breast, colorectal, liver and brain cancer, alone or in combination with other chemotherapeutic agents.14–16 Due to its structure, 5-FU interferes with nucleoside metabolism and can be incorporated into RNA and DNA, leading to cytotoxicity and tumor cell apoptosis, by inhibiting thymidylate synthase.14 There are several limitations to the use of 5-FU, including its erratic oral bioavailability, with variable gastrointestinal absorption and rapid degradation, its short plasma half-life, development of resistance and important cytotoxicity to normal cells.15,17,18 Thus, preparation of DDS containing 5-FU could improve its oral bioavailability, preventing its premature degradation and decrease its side effects, increasing concentrations at the target cell site, with lower cytotoxicity for normal cells.
The morphology and the particle size of the hosts were carried out by scanning electron microscopy (SEM) (JOEL JSM-6010LV/Braga). Textural characterization of the hosts was based on the analysis of N2 adsorption isotherms, measured at −196 °C with a Nova 4200e (Quantachrome Instruments) equipment.
The amount of loaded 5-FU was measured using thermogravimetric analysis (TGA) in a STA 409 PC/4/H Luxx Netzsch thermal analyzer. The atmosphere used was high purity air (99.99% minimum purity) with a flow rate of 50 cm3 min−1. The sample holders used were crucibles of alumina oxide, supplied by Netzsch. The samples were heated between 50 and 700 °C at 10 °C min−1 to evaluate the thermal stability.
The stability of the host structures was evaluated by FTIR and XRD analyses. In these studies, 15 mL of pH 2.0 BF solution was added to 10 mg of NaY or ETS-10 during 72 h under stirring at room temperature. The suspensions were filtered off and dried in an oven at 60 °C for 24 h, and stored in a desiccator. The pH of the suspensions was monitored during the assay. Room temperature Fourier Transform Infrared (FTIR) spectra of the parent structures in KBr pellets (2/100 mg) were measured using a ABB FTLA2000 spectrometer in the range 4000–500 cm−1 by averaging 32 scans at a maximum resolution of 8 cm−1. Powder X-ray diffraction analysis (XRD) was carried out using a Philips Analytical X-ray model PW1710 BASED diffractometer system equipped with a Cu X-ray tube (selected wavelength λCuKα = 1.54056 Å). Scans were taken at room temperature in a 2θ range between 5 and 70° with a step of 0.02°.
Cell viability was assessed using the in vitro Toxicology Assay Kit. Sulforhodamine B (SRB) based (Sigma-Aldrich, St. Louis, MO, USA). HCT-15 (7500 cells per 100 μL per well), RKO (6000 cells per 100 μL per well) and MDA-MB-468 (10000 cells per 100 μL per well) cells were seeded in 96-well plates and incubated at 37 °C in a 5% CO2 humidified atmosphere for 24 h. In order to assess the effects of the parent structures, 5-FU and DDS used and cells were incubated with increasing concentrations of the systems in culture medium. Controls were performed with culture medium alone. After an incubation period of 48 h, the spent media were removed and the plate wells were washed with 1× phosphate-buffered solution, pH 7.4 (PBS). After a fixation step with cold 10% trichloroacetic acid (TCA), cells were stained with 0.4% SRB and the incorporated dye was solubilized with 10 mM of Tris. Absorbance was monitored with a microplate reader at 570 nm with a background absorbance of 655 nm. Cell viability was determined as percentage of viability: (OD experiment/OD control) × 100 (%). One-way ANOVA, followed by Dunnett post-test were used to perform cell viability assay statistical analysis. The previous tests and 50% growth inhibition (IC50) were determined using the Graphpad Prism 5® software. The level of significance in all the statistical analysis was set at ***p < 0.001 compared to host alone. All assays were performed in triplicate from three independent experiments. The results were expressed as mean value ± SD of the triplicate assays.
The chosen 3D zeolites belong to the FAU (large pores), MFI (medium pores) and LTA (small pores) structures.24 ETS-10 is a 3D large pore structure with titanium in the framework.11 The hosts NaY (FAU) and NaA (LTA) were commercially available. However, the parent ETS-10 structure was synthesized and the parent (NH4)ZSM5 (MFI) was modified in order to obtain HZSM5 and NaZSM5. The modifications in ZSM5 host were performed to study the pore aperture effect in the loading of 5-FU. NaY was already used in a previous publication to encapsulate 5-FU and it is used here for comparison with the remaining structures.4
SEM and nitrogen adsorption analyses were performed for evaluating the average particle size and the surface area of the hosts, respectively. Fig. 1 display the SEM micrographs of some silica microporous structures. The average of the particle size obeys the following sequence, larger particles (>100 nm): NaY 700 nm,4 ZSM5 880 nm, NaA 2700 nm and ETS-10 with 2600 nm and smaller particles (<100 nm): LTL 80 nm.4
The nitrogen adsorption measurements show different surface areas for the zeolite hosts. The BET surface areas (SBET) was determined by the BET equation and the values obtained were: NaY 792 m2 g−1, LTL 566 m2 g−1, ZSM5 395 m2 g−1 and NaA 290 m2 g−1.
The 5-FU release studies were carried out with all DDS at different pH media simulating blood and gastrointestinal body fluids,25 and the stability of the host structure at pH = 2.0 was studied with NaY and ETS-10. Besides, cell viability studies were performed on HCT-15 and RKO human colon cancer cells for all hosts and DDS. However, the viability on MDA-MB-468 human breast cancer cell was tested only with the DDS based in NaY, ETS-10 (large pores) and compared with NaA (small pores).
Table 1 shows the loading of 5-FU in the silica microporous structures obtained by thermogravimetric analysis. TGA profile results are similar for all DDS with two weight losses observed; the first weight loss was related to the removal of physisorbed water of the host structure and the other one was attributed to the presence of 5-FU in the DDS.4 The presence of acetone was not detected by TGA analysis since the temperature used in the preparation of the DDS is sufficient to evaporate the solvent.26
It is evident that loading of 5-FU in the DDS is related to the porous framework of the hosts. The large pore structures, NaY and ETS-10, have higher 5-FU loading capacity than the remaining microporous structures. The medium pore structure HZSM5 presents intermediate 5-FU loading and the introduction of sodium in (NH4)ZSM5 by ion exchange, enhances a decrease in the loading of 5-FU with 6.8 mmol per ghost for NaZSM5, followed by the small pore structure NaA (3D) with 5.8 mmol per ghost.
In order to mimic the in vitro conditions of the cancer cell viability assays, 5-FU release studies from the silica microporous structures were performed in culture medium at different pH values and 37 °C, by UV/vis analysis. The release was recorded for 360 min, however the concentration remained constant after 60 min.
These studies have been conducted at pH 7.4, 5.8 and 2.0 in order to simulate the blood, intestinal and stomach fluids, respectively.25 As expected, 5-FU can be easily released from the different microporous hosts due to its small size.4 As an example, the UV/vis spectra of 5-FU release from 5-FU@NaY (Fig. S1, ESI†) and the release profiles versus time at pH 7.4 of the prepared DDS are shown in Fig. 2.
Fig. 2 Release profiles of DDS obtained in BF solution at pH = 7.4 and 37 °C, followed during 60 min. Release measurements were conducted in triplicate and the concentration values were averaged. |
The results from UV/vis spectra show that, after release from the DDS, 5-FU molecule maintain the characteristic wavelength of the drug (λ = 260 nm).27 All DDS release profiles show similar behavior, with almost complete release of the adsorbed 5-FU after 40 min, as a consequence of the rapid desorption of the drug.
The cumulative concentrations for all DDS stabilize after this time and display approximately the same value until the end of the dissolution assays (360 min). However, some differences were observed between ETS-10 and the zeolites. The release of 5-FU appears to be dependent on the 5-FU loading capacity of the host. From the large pore 3D structures, ETS-10 and NaY display the highest 5-FU release rates due to the higher amount of adsorbed drug, being ETS-10 release slightly faster (Table 1). The DDS based on NaA show the slowest rates compared to the large pore structures. The same behavior was observed for HZSM5 and NaZSM5, with the last one presenting the lowest release rates due to the introduction of sodium in the structure (Fig. 3).
These results are in line with those reported in the literature, which show that silica materials are an important class of controlled release matrices.
In the work of Larsen et al. the effect of different Si/Al ratio of Y zeolite as hosts for loading and release of 5-fluorouracil were studied and they observed a similar behavior.28 Also, the DDS based on mesoporous organo-silica nanoparticles with different drugs present the same profile of drug release29 and the mesoporous silica material SBA-15 was used to achieve immediate release of poorly soluble drug compounds such as itraconazole.30 However, the studies stated by Martens et al. show that the release of the antiseptic chlorhexidine, a large molecule with antibacterial activity, from amorphous microporous silica was fine-tuned by adapting particle size and pore diameter.31 Recently, our group also showed that the release of salicylic acid from mesoporous and microporous silica hosts is dependent of the pore size of the carriers.26
There are several mathematical kinetic models used to describe in vitro drug dissolution and drug release from particles.32–34 Weibull, first-order, Higuchi and Korsmeyer–Peppas are some of the major applied and best describing drug release models.32–34 The release profiles at pH 7.4 were fitted to these models and the Weibull model shows the best fitting (Fig. S2, ESI†). In this kinetic model two parameters were determined, the shape (“b”) and scale (“a”) parameters. The “b” parameter describe the shape of the dissolution curve and the “a” parameter represents the time dependence of the system.32–34 These parameters obtained with this model are listed in Table 2.
The “b” parameter calculated for all DDS seems to be dependent on the microporous structures of the hosts. The large pore structures, NaY and ETS-10, have the highest values with 0.35 and 0.40, respectively; followed by the medium pores with similar values and the small pores with 0.15. This parameter describes the shape of the curve as exponential (b = 1, case 1), sigmoid, S-shaped, (b > 1, case 2), or parabolic, with a higher initial slope and after that consistent with the exponential (b < 1, case 3).32–34 The obtained values are in accordance to case 3 where the release profiles of all DDS present in Fig. 2 and 3 show this behavior.
The effect of pH on drug release was also evaluated at pH 5.8 and 2.0. The lower pH medium is more likely to affect the stability of the DDS since 5-FU is a weak acid (pKa of 7.93) and the pH influences the interaction of the molecule with the surface.27 For these studies, all DDS were submitted to these different pH media, and Fig. 4 shows the results obtained for 5-FU@NaA, as an example.
As expected from 5-FU structure, even at low pH, the 5-FU molecule is not degraded since, we did not observe any shift of the characteristic wavelength of the drug. The results show that the variation of pH does not affect the release of 5-FU in the pH range of 2.0 to 7.4. The effect of pH was also studied for evaluation of the stability of the host microporous structures since the host is important in the protection of the drug in the DDS.
For this study, the large pore structures NaY and ETS-10 were selected and tested at lower pH. Besides, we also aimed to check if there was any leaching of Al3+ from NaY or Ti4+ from ETS-10. These hosts were incubated to pH 2.0 BF solution for 72 h under stirring, at room temperature. After the filtration and drying steps, the resulting solids were analyzed by FTIR and XRD. Fig. 5 displays the FTIR spectra obtained before and after NaY and ETS-10 treatment, respectively.
Fig. 5 FTIR spectra before and after treatment with pH = 2.0 BF solution of (a) NaY and (b) ETS-10, for 72 h. |
FTIR spectra obtained after treatment for both host structures show the fingerprint bands of the parent structures.35,36 Also, the principal vibrational bands of the host structures after treatment do not shift or broaden, which confirms that the pore frameworks remain unchanged. So, in all FTIR spectra, a very intense broad band at ca. 3500 cm−1 attributed to the hydroxyl groups, the ν(O–H) deformation band at 1650 cm−1 characteristic of the absorbed water and in the spectral region between 1250 and 500 cm−1, the bands corresponding to the lattice vibrations of the structures are observed.35,36
In the case of NaY zeolite, FTIR analysis allows to determine the framework Si/Al ratio using the characteristic band of the zeolite specific double ring vibration mode between 570–600 cm−1.37 From both spectra in Fig. 5a, bands at 577.1 cm−1 and 577.7 cm−1 for NaY and NaY (pH = 2.0) were identified, respectively. From these values, the calculated framework Si/Al ratio was 2.66 for NaY and 2.71 for NaY (pH = 2.0). The increase in Si/Al ratio observed is related to partial desalumination due to the contact of the zeolite structure with the lower pH solution.
XRD analysis confirms these results. The powder X-ray diffraction patterns endorses that the crystalline structure of NaY or ETS-10 does not change after the treatment. In both XRD patterns after treatment, the characteristic Miller peaks corresponding to NaY or ETS-10 were observed indicating that the host structures are not severely affected by low pH (pH 2.0). Fig. 6 displays the XRD patterns of ETS-10 and ETS-10 at pH = 2.0. From Fig. 6, the XRD patterns of both samples ETS-10 and ETS-10 (2.0) are similar and in good accordance with those reported in the literature.38,39
The relative crystallinity was estimated by the intensity of the characteristic diffraction peaks of the host structures after treatment, compared to the pattern of the parent structures, which was set to be 100% crystalline. The crystallinity obtained was 100% for ETS-10 (pH = 2.0) and 85% for NaY (pH = 2.0). These results confirm that ETS-10 is not affected by pH 2.0 and NaY is slight affected by the treatment, which is in agreement with the FTIR results.
The viability assays with microporous structures were optimized in our previous work.26 So, from a stock suspension (1.0 mg DDS per mL), sequential dilutions were made with free serum culture medium in order to get several working DDS concentrations (0.005 to 0.100 mg mL−1). The alterations in cell biomass were evaluated by the sulforhodamine B (SRB) method and were assessed after 48 h of incubation with DDS and the parent hosts.
The parent hosts did not induce reduction in cell viability in any of the cell lines studied. As an example, the ability of 5-FU released from 5-FU@ETS-10 and 5-FU@NaA to affect RKO cell viability is shown in Fig. 7. The other DDS cell viability are shown in Fig. S3 (ESI†).
The hosts titanosilicate (ETS-10) and NaA do not show cytotoxicity to the colorectal cancer cell lines studied. The results are in accordance with what we have found for in the DDS prepared with different zeolite structures as hosts with the anticancer drugs α-cyano-4-hydroxycinnamic acid (CHC) and 5-fluorouracil (5-FU), and we also studied their in vitro efficacy against colorectal carcinoma cells.4,26,40 These results are also in line with several toxicity studies which suggest that the internal surface of zeolites does not interact with the biological systems.41 However, the acidic surface characteristics could be also associated with some toxicity for specific cells. In fact, we showed that NaY zeolite induces toxicity in glioblastoma cells in all range of concentrations studied, but NaMOR zeolite did not show any toxicity to these cells. These results confirm that the zeolite toxicity is dependent on the cell type and the properties of the carrier structure. Also, in the same study, our results show that the DDS prepared with NaMOR are capable of reducing tumor size, using the in vivo chick chorioallantoic membrane (CAM) model.21
From Fig. 7, we can also see that there is an evident reduction in cell viability with 5-FU containing DDS, compared to ETS-10 or NaA (controls), with increasing concentrations of 5-FU in both DDS, for RKO cells.
Table 3 shows the IC50 values for RKO and HCT-15 cell viability after treatment with the DDS systems, compared with the drug alone.
It is clear from the results that there is a potentiation of the 5-FU when is encapsulated into the silica microporous structures in both colorectal cancer cell lines. This potentiation is dependent on the capacity of 5-FU loading, as well as on the porous framework of the hosts. For RKO cells, there is an increase in efficiency of the drug between 1.2 and 6.5-fold. The highest potentiation (6.5-fold) was obtained with the 3D structures, (ETS-10 and NaY, large pores) and HZSM5 (medium pores) hosts, which correspond to 5-FU assay concentrations of 0.02 mM.
Concerning ZSM5 structure, the reduction in pore aperture in NaZSM5 decreases 5-FU potentiation in about 4-fold when compared to 5-FU@HZSM5. The lowest potentiation obtained was for NaA host (1.2-fold), which contains 0.11 mM of 5-FU. Likewise, treatment of HCT-15 cells with the encapsulated 5-FU resulted in a potentiation of the effect of the drug from 3.1 to 6.8-fold, corresponding to 5-FU assay concentrations of 0.20 and 0.09 mM, respectively. For this cell line, the 3D structures seem to favor the potentiation of the drug, rather than the loading of 5-FU in the hosts.
The viability assays were also performed on MDA-MB-468 human breast cancer cells with the DDS based on NaY and ETS-10 (large pores) and compared with NaA (small pore). In addition, these hosts are not toxic for this cell line (Fig. S4, ESI†) and the DDS based in these hosts were very efficient in the decrease of the cell viability (Table 4). Importantly, inclusion of 5-FU in the DDS, increased substantially the sensitivity of the breast cancer cells to 5-FU, which are more resistant than the colon cancer cells.
Cell line | MDA-MB-468 | |
---|---|---|
IC50 (mM) | Potentiation | |
5-FU | 3.80 | — |
5-FU@NaY | 0.38 | 10.0 |
5-FU@NaA | 0.37 | 10.2 |
5-FU@ETS-10 | 0.19 | 19.9 |
For MDA-MB-468 cells, the best DDS was obtained with ETS-10 with higher 5-FU loading. The potentiation obtained is double compared to the zeolite systems, which display the same potentiation among them, despite different loadings (Table 1).
The potential of these host structures will depend on the design and delivery route to be used. While smaller particles (<100 nm, LTL zeolite) would be suitable for systemic administration, e.g. intra venous or inhalation delivery, larger particles (>100 nm, NaY, NaA and ZSM5 zeolites, and ETS-10) will have potential for topical delivery (Fig. 1). Besides systemic treatment, 5-FU is also used topically for the treatment of non-melanoma skin cancer (e.g. superficial basal cell carcinoma) or even other skin pathological conditions such as actinic or solar keratosis.42,43
Footnote |
† Electronic supplementary information (ESI) available. See DOI: 10.1039/c7ra01028a |
This journal is © The Royal Society of Chemistry 2017 |