Development of glycosynthases with broad glycan specificity for the efficient glyco-remodeling of antibodies

Sachin S. Shivatare; Lin-Ya Huang; Yi-Fang Zeng; Jung-Yu Liao; Tsai-Hong You; Shi-Yun Wang; Ting Cheng; Chih-Wei Chiu; Ping Chao; Li-Tzu Chen; Tsung-I Tsai; Chiu-Chen Huang; Chung-Yi Wu; Nan-Horng Lin; Chi-Huey Wong

doi:10.1039/C8CC03384F

Development of glycosynthases with broad glycan specificity for the efficient glyco-remodeling of antibodies†

Sachin S. Shivatare,

^a Lin-Ya Huang,^a Yi-Fang Zeng,^a Jung-Yu Liao,^a Tsai-Hong You,^a Shi-Yun Wang,^a Ting Cheng,^b Chih-Wei Chiu,^a Ping Chao,^a Li-Tzu Chen,^a Tsung-I Tsai,

^c Chiu-Chen Huang,^a Chung-Yi Wu,

*^b Nan-Horng Lin*^a and Chi-Huey Wong

*^bc

Author affiliations

* Corresponding authors

^a CHO Pharma Inc., 18F, Building F, No. 3, Park Street, Nangang District, Taipei-11503, Taiwan
E-mail: nhlin@chopharma.com.tw

^b Genomics Research Center, Academia Sinica, 128 Academia Road, Section 2, Nankang District, Taipei 115, Taiwan
E-mail: cyiwu@gate.sinica.edu.tw, chwong@gate.sinica.edu.tw

^c Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA

Abstract

The first systematic investigation of the effect of high mannose, hybrid, and bi- and tri-antennary complex type glycans on the effector functions of antibodies was achieved by the discovery of novel Endo-S2 mutants generated by site-directed mutagenesis as glycosynthases with broad substrate specificity.

Chemical Communications

Development of glycosynthases with broad glycan specificity for the efficient glyco-remodeling of antibodies†

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Development of glycosynthases with broad glycan specificity for the efficient glyco-remodeling of antibodies

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