Correction: A benzylic linker promotes methyltransferase catalyzed norbornene transfer for rapid bioorthogonal tetrazine ligation

Abstract

Correction for ‘A benzylic linker promotes methyltransferase catalyzed norbornene transfer for rapid bioorthogonal tetrazine ligation’ by F. Muttach et al., Chem. Sci., 2017, DOI: 10.1039/c7sc03631k.

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The authors regret that Fig. 4 is incorrect in the original manuscript. In Fig. 4c the chemical structure and mass spectrum of the norbornene-modified adenosine was shown instead of the 2′-deoxyadenosine. The correct figure and caption are displayed below.

Fig. 1 Norbornene modification of pBR322 plasmid DNA using the N⁶-adenine MTase M. TaqI. (A) Scheme for the functionalization of plasmid DNA using norbornene-modified AdoMet analog 1b. (B) Fluorescence labeling of plasmid DNA via norbornene-modification followed by labeling with TAMRA-tetrazine and linearization of the plasmid using BamHI. Bands were resolved on a 1% agarose gel (100 V, 50 min), the gel was stained using ethidium bromide and scanned on a Typhoon FLA9500 laser scanner. (C) Mass spectrometric analysis of N⁶-norbornene-modified oligonucleotides. A DNA oligonucleotide was subjected to enzymatic norbornene-modification, followed by digestion using nuclease P1 and dephosphorylation using FastAP (ThermoFisher Scientific). Expected mass for C₂₆H₃₁N₆O₄⁺ = 491.2401 [M + H]⁺, found: 491.2400. M: GeneRuler 1 kb DNA ladder (ThermoFisher).

The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers.

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