Issue 78, 2020
From the journal:

Chemical Communications

Dephosphorylation-directed tricyclic DNA amplification cascades for sensitive detection of protein tyrosine phosphatase†

Yueying Li,‡a   Shuli Sun,‡a   Xiaorui Tian,‡a   Jian-Ge Qiu,b   BingHua Jiang,b   Li-juan Wang*a  and  Chun-yang Zhang ORCID logo *a  

* Corresponding authors

a College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China
E-mail: cyzhang@sdnu.edu.cn, wlj-sdnu@foxmail.com
Fax: +86 0531-82615258
Tel: +86 0531-86186033

b Academy of Medical Sciences, Zhengzhou University, Zhengzhou 450000, China

Abstract

We develop a new fluorescence method for the sensitive detection of protein tyrosine phosphatase 1B (PTP1B) based on dephosphorylation-directed tricyclic DNA amplification cascades. This method exhibits good specificity and high sensitivity with a detection limit of 0.24 pM. Moreover, it can be applied for kinetics analysis, inhibitor screening, and the accurate detection of PTP1B in a variety of cancer cells.

Graphical abstract: Dephosphorylation-directed tricyclic DNA amplification cascades for sensitive detection of protein tyrosine phosphatase

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Article information

DOI
https://doi.org/10.1039/D0CC04714G
Article type
Communication
Submitted
08 Jul 2020
Accepted
03 Sep 2020
First published
03 Sep 2020

Chem. Commun., 2020,56, 11581-11584

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Dephosphorylation-directed tricyclic DNA amplification cascades for sensitive detection of protein tyrosine phosphatase

Y. Li, S. Sun, X. Tian, J. Qiu, B. Jiang, L. Wang and C. Zhang, Chem. Commun., 2020, 56, 11581 DOI: 10.1039/D0CC04714G

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