Issue 2, 2021

A novel ratiometric fluorescent probe for the detection of mitochondrial pH dynamics during cell damage

Abstract

A sensitive fluorescent probe (E)-4-(3-(benzo[d]thiazol-2-yl)-4-hydroxy-5-methylstyryl)-1-methylpyridin-1-ium iodide (HBTMP) for the monitoring of pH in mitochondria was rationally exploited. This novel probe exhibited remarkable pH-dependent behavior in the linear range of 5.5–8.0, along with a pKa value of 6.829 ± 0.02627. A large Stokes shift of 205 nm was obtained. This fluorescent probe demonstrated good biocompatibility and high sensitivity for detecting the dynamic changes in mitochondrial pH in living cells and zebrafish. The results of the CCCP (m-chlorophenyl hydrazone) treatment experiment indicated that the probe can effectively monitor changes in mitochondrial pH caused by cell damage.

Graphical abstract: A novel ratiometric fluorescent probe for the detection of mitochondrial pH dynamics during cell damage

Supplementary files

Article information

Article type
Paper
Submitted
21 Jun 2020
Accepted
21 Sep 2020
First published
14 Nov 2020

Analyst, 2021,146, 620-627

A novel ratiometric fluorescent probe for the detection of mitochondrial pH dynamics during cell damage

G. Fu, G. Yin, T. Niu, W. Wu, H. Han, H. Chen and P. Yin, Analyst, 2021, 146, 620 DOI: 10.1039/D0AN01240H

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