Issue 4, 2021

Fluorogenic probes for mitochondria and lysosomes via intramolecular photoclick reaction

Abstract

The tetrazole-based photoclick chemistry has attracted considerable attention in virtue of its good biocompatibility, exclusive molecular reaction, and spatiotemporally controllable properties. Using this photoclick reaction, we designed an in situ, real-time fluorescence imaging system that targeted mitochondria and lysosomes in a spatiotemporally controllable manner. Upon irradiation, the pyrazoline fluorophore was generated in situ by the intramolecular tetrazole-alkene cycloaddition reaction (“photo-click chemistry”). This strategy exhibits features such as fast response, high efficiency, strong fluorescence intensity without background and superior stability. In addition, by integrating with an organelle-specific group, it has a good application for subcellular targeting imaging. Furthermore, the photo-responsive moiety Tet facilitates the probes, Mt-Tet and Ly-Tet, for the super-resolution imaging of subcellular structures.

Graphical abstract: Fluorogenic probes for mitochondria and lysosomes via intramolecular photoclick reaction

Supplementary files

Article information

Article type
Paper
Submitted
07 Oct 2020
Accepted
07 Dec 2020
First published
09 Dec 2020

Analyst, 2021,146, 1369-1375

Fluorogenic probes for mitochondria and lysosomes via intramolecular photoclick reaction

S. Liu, H. Su, L. Bu, J. Yan, G. Li and J. Huang, Analyst, 2021, 146, 1369 DOI: 10.1039/D0AN01982H

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