Preparation of a broad-specific monoclonal antibody and development of an immunochromatographic assay for monitoring of anthranilic diamides in vegetables and fruits

Abstract

Anthranilic diamide insecticides, including chlorantraniliprole (CHL), cyantraniliprole (CYA), cyclaniliprole (CYC), and tetrachlorantraniliprole (TEA), are widely used in agricultural production, resulting in potential risk to human health. In this study, we designed novel haptens for the preparation of a broad-specific monoclonal antibody (mAb) against CHL, CYA, CYC, and TEA simultaneously. The mAb 4F5 we produced belonged to the IgG1 subtype and had 50% inhibition concentration (IC₅₀) values ranging from 0.06 to 0.19 ng mL⁻¹ for all four anthranilic diamides. We further developed a mAb-based lateral flow immunochromatographic strip for the detection of anthranilic diamides in vegetables and fruits. Under optimal conditions, the strip provided cut-off values of 2.5, 5, 10, and 10 ng g⁻¹ for CHL, CYA, CYC, and TEA, respectively, within 15 min. Collectively, these data show that the proposed strip assay is a convenient and practical method for the rapid screening of anthranilic diamides in food samples.