Biomimetic peptide nanoparticles participate in natural coagulation for hemostasis and wound healing

Abstract

Uncontrolled hemorrhage is a major problem both in surgical intervention and after trauma. Herein, we design an in situ constructable peptide network, mimicking and participating in the native coagulation process for enhanced hemostasis and wound healing. The network consists of two peptides including C₆KL, mimicking platelets and C₆KG, mimicking fibrin. The C₆KL nanoparticles could bind to the collagen at the wound site and transform into C₆KL nanofibers. The C₆KG nanoparticles could bind to GPIIb/IIIa receptors on the surface of activated platelets and transform into C₆KG nanofibers. The in situ formed peptide network could interwind platelets, fibrin and red blood cells, causing embolism at the wound site. In a lethal femoral artery, vein, and nerve cut model of rats, the amount of bleeding was reduced to 32.8% by C₆KL and C₆KG with chitosan/alginate. The biomimetic peptides show great clinical potential as trauma hemostatic agents.