Issue 18, 2022

Acoustofluidic cell micro-dispenser for single cell trajectory control

Abstract

The precise manipulation of individual cells is a key capability for the study of single cell physiological characteristics or responses to stimuli. Currently, only large cell populations can be transferred with certainty using expensive and laborious flow cytometry platforms. However, when approaching small populations of cells, this task becomes increasingly challenging. Here, we report an effective acoustofluidic micro-dispenser, utilising surface acoustic waves (SAWs), with the ability to trap and release cells on demand, which when combined with an external valve can guide the trajectory of individual cells. We demonstrate single cell trap and release with a single cell trapping effectiveness of 74%, enabling the capability of dispensing a highly controlled amount of cells without any harmful effects. This device has the potential to be easily integrated into a wide range of analytical platforms for applications such as single cell fluorescent imaging and single cell proteomic studies.

Graphical abstract: Acoustofluidic cell micro-dispenser for single cell trajectory control

Supplementary files

Article information

Article type
Paper
Submitted
07 Mar 2022
Accepted
15 Aug 2022
First published
15 Aug 2022

Lab Chip, 2022,22, 3533-3544

Acoustofluidic cell micro-dispenser for single cell trajectory control

C. Richard, C. Devendran, D. Ashtiani, V. J. Cadarso and A. Neild, Lab Chip, 2022, 22, 3533 DOI: 10.1039/D2LC00216G

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