Split G-quadruplex-programmed label-free CRISPR-Cas12a sensing system

Mengting Xu; Yuedong Zhu; Yan Zhang; Bin Gong; Yuliang Kang; Gaoxing Su; Yanyan Yu

doi:10.1039/D3CC01239E

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Split G-quadruplex-programmed label-free CRISPR-Cas12a sensing system†

Mengting Xu,‡^a Yuedong Zhu,‡^a Yan Zhang,^a Bin Gong,^a Yuliang Kang,^a Gaoxing Su

*^a and Yanyan Yu

*^a

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* Corresponding authors

^a School of Pharmacy, Nantong University, Nantong, Jiangsu 226001, China
E-mail: yuyanyan@ntu.edu.cn, sugaoxing@ntu.edu.cn

Abstract

A split G-quadruplex (G4)-programmed Cas12a platform was established, validated, and optimized. The split G4 motif was recruited as substrate for Cas12a, and the label-free sensing platform provided a concentration-dependent response towards the input target. Furthermore, exosomal surface proteins from cultured cancer cells and clinical samples were detected and profiled.

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Article information

DOI: https://doi.org/10.1039/D3CC01239E
Article type: Communication
Submitted: 13 Mar 2023
Accepted: 17 May 2023
First published: 31 May 2023

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Chem. Commun., 2023,59, 7615-7618

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Split G-quadruplex-programmed label-free CRISPR-Cas12a sensing system

M. Xu, Y. Zhu, Y. Zhang, B. Gong, Y. Kang, G. Su and Y. Yu, Chem. Commun., 2023, 59, 7615 DOI: 10.1039/D3CC01239E

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Chemical Communications

Split G-quadruplex-programmed label-free CRISPR-Cas12a sensing system†

Abstract

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Split G-quadruplex-programmed label-free CRISPR-Cas12a sensing system

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