Enhancing the catalytic efficiency and stability of photoenzymes using hydrogen-bonded organic framework material HOF-101

Abstract

The development of highly efficient artificial photoenzyme catalytic systems, inspired by natural photosynthesis, has attracted significant attention. In this study, we utilized the hydrogen-bonded organic framework material, HOF-101, to embed tetrakis (4-carboxyphenyl) porphine (TCPP), [Cp*Rh(bpy)H₂O]²⁺ (M), and lactate dehydrogenase (LDH) in situ, creating a nanoscale hydrogen-bonded photoenzyme-catalyzed platform (TML@HOF-101). HOF-101, along with TCPP and M, constitutes a heterojunction photocatalyst that synergistically catalyzes the conversion of pyruvate to lactate with the help of lactate dehydrogenase. TML@HOF-101 exhibited excellent catalytic activity and stability, producing 185.2 μM lactic acid within 1 h and maintaining 98% activity over 10 consecutive batches. This is the first report on the use of HOF materials for photoenzyme-catalyzed reactions, highlighting the huge potential of HOF materials in this field.