Quantitative determination of the binding capabilities of individual large-ring cyclodextrins in complex mixtures

Abstract

Large-ring cyclodextrins (CDs) are a comparatively unexplored family of macrocycles. We use high-resolution ¹H–¹³C HSQC NMR experiments to resolve the anomeric signals of at least 13 different size CDs in a mixture. Using a single titration experiment, we can quantify the individual binding capabilites of these structurally-related hosts, avoiding the need for cumbersome isolation.