Shasha
Yang
ab,
Luz Estefanny
Quispe Cardenas
ab,
Athkia
Fariha
a,
Nada
Shetewi
c,
Victor
Melgarejo Cazares
ab,
Nanyang
Yang
a,
Lewis
McCaffrey
d,
Nicole
Wright
d,
Michael R.
Twiss
e,
Siwen
Wang
a,
Stefan J.
Grimberg
a and
Yang
Yang
*a
aDepartment of Civil and Environmental Engineering, Clarkson University, Potsdam, New York 13699, USA. E-mail: yanyang@clarkson.edu
bInstitute for a Sustainable Environment, Clarkson University, Potsdam, New York 13699, USA
cDepartment of Chemical Engineering, The Cooper Union, New York, New York 10003, USA
dNew York State Department of Environmental Conservation, 625 Broadway, Albany, New York 12333, USA
eFaculty of Science, Algoma University, Sault Ste. Marie, Ontario P6A 2G4, Canada
First published on 30th July 2024
Harmful algal blooms (HABs) are an emerging threat to ecosystems, drinking water safety, and the recreational industry. As an environmental challenge intertwined with climate change and excessive nutrient discharge, HAB events occur more frequently and irregularly. This dilemma calls for fast-response treatment strategies. This study developed an electrochemical ozonation (ECO) process, which uses Ni–Sb–SnO2 anodes to produce locally concentrated ozone (O3) and hydroxyl radicals (·OH) to achieve ∼100% inactivation of cyanobacteria (indicated by chlorophyll-a degradation) and removal of microcystins within 120 seconds. More importantly, the proof-of-concept evolved into a full-scale boat-mounted completely mixed flow reactor for the treatment of HAB-impacted lake water. The single-pass treatment at a capacity of 544 m3 d−1 achieved 62% chlorophyll-a removal with an energy consumption of <1 Wh L−1. Byproducts (e.g., chlorate, bromate, trihalomethanes, and haloacetic acids) in the treated lake water were below the regulatory limits for drinking water. The whole effluent toxicity tests suggest that ECO treatment at 10 mA cm−2 posed certain chronic toxicity to the model crustacean invertebrate (Ceriodaphnia dubia). However, the treatment at 7 mA cm−2 (identified as the optimum condition) did not increase toxicity to model invertebrate and fish (Pimephales promelas) species. This study is a successful example of leveraging fundamental innovations in electrocatalysis to solve real-world problems.
Water impactThis study developed an innovative electrochemical ozonation (ECO) process to mitigate harmful algal blooms. The ECO process oxidizes water to generate ozone, which rapidly inactivates cyanobacteria and degrades toxins within minutes. Its high efficacy was demonstrated in both laboratory settings and pilot-scale real-world lake applications, showcasing low energy consumption and minimal byproduct formation. |
Chemical oxidation (using chlorine, permanganate, H2O2, etc.)5–7 and advanced oxidation processes (UV/H2O2 and UV/chlorine)8 are promising for HAB mitigation at drinking water treatment works. However, they are less practical for on-site lake remediation due to the uncertain efficacy limited by hydrological conditions and concerns about the environmental impact of chemicals.
There is a critical need for in situ treatment technology that can proactively curb HABs at the early stage and precisely eliminate algae plumes and cyanotoxins in the impacted high-value areas (lake shores, public beaches, etc.). Since the bloom is usually concentrated in the top 0–1 m of the water column,9,10 we envision that mobile devices with pump-and-treat functionality could realize the precise treatment of the plume, instead of the whole water body, for maximum cost-effectiveness. Electrochemical oxidation (EO)-based technologies, with the advantages of small footprint, high efficiency, and additive-free operation, could be the best fit. Previously, the EO treatment of algae and microcystins equipped with anodes made of boron-doped diamond,11 metal oxide (RuO2 and IrO2),12,13 and graphite14 was investigated at the lab scale. We advanced the EO process by developing Ti4O7 filter anodes with pore sizes of 24–53 μm for the treatment of HAB-impacted lake water at both lab and full scales.15 The Ti4O7 filter anodes oxidize chloride in lake water to chlorine to inactivate algae cells and oxidize cyanotoxins. The flow-through operation (by pumping water out of the filter anodes immersed in the plume) significantly promoted convective mass transfer and thereby achieved higher treatment performance than homogenous chlorination. Though the scaled-up demonstration at a capacity of 110 m3 d−1 was successful, two limitations were identified: (1) the chlorine yield, which is associated with treatment performance, depends on chloride concentrations in lake water. (2) The micropores of the filter anode create large pressure drops ahead of the pump. The treatment capacity is limited by the pump suction head, which is significantly smaller than the lift head. The pumps were also vulnerable to damage by cavitation.
This study aims to address both engineering and fundamental challenges. The anode configuration was transformed from microporous cartridge filters to an array of mesh electrodes. Correspondingly, as will be discussed below, the treatment operation was changed from pumping water out of the submerged filter anode to pumping water through the mesh electrode array in a boat-mounted reactor to gain a larger treatment capacity (i.e., the flow rate is no longer limited by the pump suction head). The increase in design treatment capacity necessitates the use of more efficient electrodes that can produce various oxidants in addition to free chlorine, thereby removing the constraint of chloride concentration in lake water on treatment performance. In this regard, electrochemical ozone (O3) evolution (ECO) is a promising target reaction,16 as this reaction uses water molecules as the only feedstock (3H2O → O3(g) + 6H+ + 6e−, E0 = 1.51 VRHE).
Electrode materials made of Pt, PbO2, and boron-doped diamond (BDD) are able to produce O3.16 In addition to the cost concern, these electrodes have to be operated at high current densities (50-1000 mA cm−2) and acidic electrolyte (H2SO4 or HClO4) to achieve noticeable ozone production.17,18 The harsh conditions are less applicable to treating environmental water. Moreover, operations at high current density may promote the formation of halogenated byproducts.19 Recently, nickel-doped antimony tin oxide (NATO: Ni–Sb–SnO2) emerged as a more reactive electrode for O3 production. It could be operated at lower current densities (100–200 mA cm−2) for substantial current efficiencies (10–30%) in acidic electrolytes.20,21 Note that most of the studies on the ECO have been carried out in acidic electrolytes. This is because the apparent O3 evolution rate decreases with the increased pH,16 due to the promoted transformation of O3 to hydroxyl radical (·OH) and the decreased O3 solubility at elevated pH.22,23 Despite the potential compromise of O3 yield, recent environmental applications demonstrated that NATO could effectively produce O3 and the derivative ·OH in electrolytes and latrine wastewater at near-neutral pH, facilitating organic degradation and disinfection.24,25
This is the first study to explore the fundamentals, applications, and environmental implications of NATO electrodes in HAB mitigation. The significant advancement brought by NATO is the shift from using free chlorine to O3 and ·OH as the reactive species, enabling treatment performance to be independent of chloride concentration in lakes. In addition to process innovation and scaled-up demonstration, this study provides comprehensive evaluations of the environmental implications, covering process impacts on byproduct formation and effluent toxicity on an invertebrate and fish.
The NATO anode comprises NATO out-layer coating (1 mg cm−2 projected area) and ATO base coating (1 mg cm−2 projected area) on Ti mesh, while the ATO anode only contains the ATO base coating. The dual-layer design of the NATO anode bestows the electrode with superior durability and O3 yield. Details were provided in our previous publication.24 The full-scale electrode array contains 20 pieces of mesh NATO anode (50 × 50 cm; 0.5 × 0.8 mm rhombus openings) sandwiched by 21 pieces of perforated stainless steel sheet cathode (50 × 50 cm; round opening at Ø 3 mm) at an interspace of 0.5 cm. The full-scale mesh NATO anodes were manufactured by Square One Coating Systems following the same sol–gel coating + calcination procedure described above.
Free chlorine was measured by a portable Hach DR900 colorimeter (HACH, CO) using a DPD (N,N-diethyl-p-phenylenediamine) reagent. Dissolved O3 concentrations were determined by the indigo method on a NanoDrop OneC spectrophotometer (Thermo Fisher Scientific).26 When measuring electrochemical O3 evolution in the presence of chloride, the anodically generated free chlorine was masked by 1 M malonic acid.24 Trihalomethanes (THMs) and haloacetic acids (HAAs) were measured by gas chromatography/mass spectrometry (GC/MS) following EPA Method 624.1. Inorganic anions were analyzed by ion chromatography (Thermo Dionex Integrion HPIC).27 The whole effluent toxicity tests on Pimephales promelas and Ceriodaphnia dubia were independently conducted by AquaTOX Research, Inc., following EPA Methods 2000.0 and 2002.0, respectively (EPA-821-R-02-013).
ERHE = Emeasured + 0.0591 × pH | (1) |
We adopted the Ni dopant level of 1 at% (Ni/(Ni + Sb + Sn)) as optimized in previous studies.29,30 The Ni doping shifts the CV hysteresis toward lower potentials. The shift of onset potentials from 2.48 V for ATO to 2.38 V for NATO and the rise of the current response can be attributed to the enhanced evolution of oxygen and O3.20 In the lake water treatment, the mesh electrodes were operated at current densities between 6–10 mA cm−2 projected area, which leads to anodic potentials around 2.6–2.7 VRHE (Fig. 1a), surpassing the thermodynamic criteria for ·OH radical evolution (·OH/H2O: 2.7 VNHE).31
Electrolysis using ATO could not generate O3. In contrast, the NATO generated dissolved O3 in PBS electrolyte in the presence or absence of Cl− (Fig. 1b). The evolution rate and current efficiency in PBS electrolyte at 10 mA cm−2 are 0.0078 mmol m−2 s−1 and 4.5%, respectively. Chlorine evolution capability was tested in PBS electrolyte amended with 1 mM Cl−, a typical Cl− concentration found in lake waters treated in this study (Table S1†). NATO and ATO demonstrated comparable chlorine evolution rates of 0.022 and 0.024 mmol m−2 s−1, respectively, at 10 mA cm−2 (Fig. S1†). The electrochemical reactive surface area (ECSA) of the NATO mesh anode was measured by the double-layer capacitance method.32 The capacitance is measured as 16.64 mF, corresponding to an ECSA of 443.6 cm2 (Fig. S2†). The estimated ECSA is 8.9 times larger than the projected surface area (50 cm2).
The stability of the NATO electrode was previously investigated by the accelerated lifetime test.24 According to the established lifetime prediction model, the NATO anode operated at the optimum current density of 7 mA cm−2 (see below) has an expected lifetime of 9800 h. The leaching of metal ions, for example, Sb, is only significant (<0.05% loss of the mass loading) after 90 min electrolysis.24 This explains the results observed in this study that Ni, Sb, and Sn were not detected (by inductively coupled plasma mass spectrometry with a detection limit of 1 μg L−1) after 3 min electrolysis when >99% Chl-a was achieved (to be discussed in the next section and Fig. 2).
This study uses extracted chlorophyll-a (Chl-a) fluorescence as an indicator of cyanobacteria viability. Chl-a cannot maintain its structural integrity and light-adsorbing properties after cell damage. It is commonly used as an indicator to quantify the viable cell concentration.36–39 We demonstrated clear linear correlations between optical density (a standard index for estimating the concentration of cells in a suspension) and Chl-a concentrations in Synechococcus elongatus and Microcystis aeruginosa cultures (Fig. S3†). These data support the feasibility of using Chl-a concentration to represent viable cell density. More importantly, under the context of lake water remediation, Chl-a concentration is a straightforward algae proxy adopted by the World Health Organization and regulators. For example, the New York State Department of Environmental Conservation (NYSDEC) uses pigment concentration >25 μg L−1 as one of the criteria of HAB rather than cell density.40 Therefore, we use the degradation of Chl-a as the performance metric for HAB mitigation.
The tests of inactivation of Synechococcus elongatus were first performed in PBS electrolyte (pH = 7.7 throughout the reactions) at a current density of 10 mA cm−2. Note that both ATO and NATO have sufficiently high anodic potentials of ∼2.7 VRHE to produce ·OH (Fig. 1a). It was found that ATO showed negligible reactivity in Chl-a removal, and NATO noticeably outperformed ATO (Fig. 2a). The poor performance of ATO suggests that surface-bound ·OH generated from the Sb–SnO2 sites is less efficient for algae inactivation. Given that both NATO and ATO share Sb–SnO2 as the primary catalyst component, except NATO containing an additional 1 mol% Ni dopant, the yield of surface-bound ·OH on the Sb–SnO2 sites of NATO should be comparable to that of ATO. However, NATO possesses the additional ability to produce O3, which can be partially transformed into ·OH in the water phase. Consequently, the superior performance of NATO should be attributed to the combined effects of O3 and its derivative ·OH.
O3 played a key role in algae inactivation, evidenced by the consumption of O3 (i.e., lower residual O3 concentrations) during the electrolysis in PBS electrolyte spiked with Synechococcus elongatus (Fig. 1b). We believe that both O3 and derivative ·OH contributed to algae inactivation, but it is challenging to quantify their contribution. This is because the conversion of O3 to ·OH is only pronounced when pH > 9,23 which means O3 and ·OH co-exist in most of the treatment scenarios (pH 7–8) demonstrated in this study. Moreover, the ECO process employs a distinct O3 dosing mechanism compared to ozonation. Thus, the strategy of adding a radical quencher to investigate direct oxidation by O3 alone is not suitable for investigating the ECO process. Specifically, the O3 evolution on NATO requires the combination of surface-bound ·OH with O2 to form ·HO3, then O3.20,41 Therefore, the addition of tert-butyl alcohol (TBA) to scavenge ·OH also halted the O3 production (below the detection limit of 0.1 mg L−1) and consequently Chl-a removal by NATO was retarded in the presence of 100 mM TBA (Fig. 2b). Though we were unable to determine whether O3 and secondary product ·OH contributed the most, the electrochemical production of O3 is the driving force of the treatment process. Therefore, in the following content, we will continue to use the term ECO to distinguish this process from other radical-mediated advanced oxidation processes.
In the presence of Cl−, NATO could produce free chlorine and O3. If chlorine made a significant contribution to Chl-a removal, then the Chl-a removal kinetics in PBS with Cl− should be faster than PBS without Cl−. However, our observation suggests otherwise. The presence of up to 3 mM Cl− (a high record in lakes of the United States42) did not further accelerate Chl-a removal (Fig. 2b; w/ Cl−vs. PBS). Previously, we determined the pseudo-first-order rate constant of Chl-a in Synechococcus elongatus and free chlorine as 0.027 L (mg−1 min−1).43 With the presence of 1 mM Cl−, the time-average free chlorine concentrations produced by NATO at 10 mA cm−2 within 120 s electrolysis should be ∼7 mg L−1 (Fig. S1†). If free chlorine is responsible for Chl-a removal, the half-life of the reaction (t1/2) should be 220 s instead of the 48 s shown in Fig. 2b. Therefore, we conclude that even in the presence of chloride, O3 and derivative ·OH are the two primary reactive species for cyanobacteria inactivation by NATO. The treatment performance of ATO can be enhanced in the presence of Cl− due to the production of free chlorine. However, as shown in Fig. 2c, a comparison of Chl-a removal kinetics in PBS with 1 mM Cl− at 10 mA cm−2 indicates that NATO (t1/2 ≈ 60 s) still significantly outperforms ATO (t1/2 ≈ 160 s).
The efficacy of ECO was compared with homogeneous ozonation (Fig. 2d). The homogeneous ozonation at 1.5 mg L−1 O3 achieved Chl-a removal kinetics similar to ECO. In contrast, ECO generated 0.48 mg L−1 O3 at 120 s, corresponding to a time-average [O3] of 0.24 mg L−1. ECO produced less residual bulk [O3] (0.24 vs. 1.5 mg L−1) to realize faster Chl-a removal kinetics than homogeneous ozonation. Therefore, the faster cyanobacteria inactivation could be attributed to the locally concentrated O3 produced at the vicinity of the NATO anode, and the regional O3 concentration is equivalent to ∼1.5 mg L−1.
The ECO treatment of two cyanobacteria strains individually spiked in PBS electrolyte with 1 mM Cl− shows that Microcystin aeruginosa and Synechococcus elongatus have similar degradation kinetics (Fig. 3a). This similarity is attributed to their identical Gram-negative cell wall structures,35 suggesting that the inactivation mechanism is likely due to cell wall damage. After elucidating the reaction mechanism, the treatment tests were operated in more field-like scenarios. In most of the field tests, the ECO full-scale system was operated at 7 mA cm−2 (see discussion below). ECO at 7 mA cm−2 is equally efficient for the inactivation of Microcystis aeruginosa (Fig. 3b) and Synechococcus elongatus (Fig. S4†) in PBS amended with 1 mM Cl−. The treatment was further operated in Lake Neatahwanta water (collected before the HAB season and filtered to exclude background cyanobacteria and MC-LR) spiked with Microcystis aeruginosa. The Chl-a removal kinetics were slightly retarded by the water matrices, but the >90% removal efficiency can still be achieved after 180 s electrolysis.
The lake water and PBS electrolyte spiked with Microcystis aeruginosa culture contains both intra- and extracellular MC-LR. In both media, intra-cellular MC-LR was degraded slower than extra-cellular MC-LR (Fig. 3c). This is because the destruction of intra-cellular MC-LR requires the cell structure to be broken down first.
The bench-scale treatability study concluded that the ECO process is efficient for the removal of cyanobacteria and MC-LR at minute-level retention time. The remaining roadblock toward field development is understanding the environmental implications of the ECO technology. Given the fast Chl-a degradation kinetics, we hypothesize that significant cyanobacteria inactivation could be achieved before the yield of byproducts. Concern about the formation of inorganic byproducts was first addressed in the bench-scale study under field-like conditions. Other environmental aspects (organic byproducts and eco-toxicity) were investigated in the field application (see the following content). Chlorate (ClO3−) was identified as the product stemming from the electrochemical oxidation of Cl−. Fig. S5† shows the evolution of ClO3− in PBS electrolyte amended with 1 mM Cl− and lake water containing ∼1 mM Cl− at 10 mA cm−2. The results indicate that the evolution of ClO3− was suppressed in the lake water compared with electrolysis in PBS electrolytes, possibly due to the depletion of free chlorine by reacting with matrice components. As will be discussed below, the full-scale ECO reactor was operated at a Chl-a removal efficiency of ∼40%, corresponding to a treatment duration of 60 s in batch mode. In this scenario, the [ClO3−] was below the detection limit (5 μg L−1) and the World Health Organization (WHO) guideline of 0.7 mg L−1.44
Bromate is a byproduct derived from the ozonation of bromide (Br−).45 We do not believe this is a concern in the lake water treatment in this study because Br− was not detected in the lake water of two test sites (Table S1†). To address the concern about the future applications in treating Br− containing water, we investigated the formation BrO3− in the ECO treatment of PBS electrolyte containing 0.2 mg L−1 Br−, a concentration reported in some surface water.46 The results show that, throughout the 180 s ECO treatment process at 7 mA cm−2, the dominant product is HOBr. Formation of BrO3− was not detected (Fig. S6†). This finding highlights the advantage of ECO over homogeneous ozonation as the former yields less bulk O3 to form BrO3−.
The bench-scale investigation concludes that (1) ECO effectively removes cyanobacteria and cyanotoxins at short, minute-level retention times; (2) the reaction is built upon the locally concentrated O3 produced at the NATO anode. The efficacy is independent of chloride concentration, which broadens the treatment scenarios in fresh waters.
The electrode array has a total projected anode area of 10 m2. Applying 600–1000 A total current on the electrode array resulted in current densities of 6–10 mA cm−2 with a cell voltage ranging from 12–20 V. In operation, water passing through the electrode mesh incurred efficient mass transfer. We have conducted preliminary tests in the field to show that the Chl-a concentrations in samples collected from the mid-height sampling port (representing bulk concentration) and outlet were the same (Fig. S8†). Therefore, the reactor can be considered a completely mixed-flow reactor (CMFR).
Based on the bench-scale batch reactions (data set of “PBS w/ 1 mM Cl−” in Fig. 2b and S4†), the pseudo-first-order rate constants (kECO's) at 7 and 10 mA cm−2 are 0.026 and 0.061 s−1, respectively. When transforming the reaction from batch to CMFR, the Chl-a removal efficiency can be estimated by
Removal efficiency (%) = (1 − C/C0) × 100% = 1 − 1/(1 + τkECO) | (2) |
The performance of the full-scale system was validated in the treatment of algal blooms that occurred in Lake Neatahwanta (43°18′30′′N 76°26′14′′W) and Oneida Lake (43°10′25′′N 75°55′50′′W) in New York state. Lake Neatahwanta was treated in the midst of an algal bloom with 105 μg L−1 Chl-a and 3 μg L−1 MC-LR, while tests on Oneida Lake happened in the early blooming stage with Chl-a concentration of 5.4 μg L−1 (Table S1†). Each field deployment lasted for one day, with 9 to 12 pairs of influent and effluent samples taken. Tests in Oneida Lake were performed at current densities of 6–7 mA cm−2, while those in Lake Neatahwanta were conducted at 7–10 mA cm−2. As shown in Fig. 4c, treatment operated at 7 and 10 mA cm−2 achieved Chl-a removal efficiency of 39% and 62%, respectively, approaching the predicted values based on the CMFR model. More importantly, plotting removal efficiency data against specific energy consumption (Wh L−1) shows that the field test performances align well with the bench-scale data set. The convergence of laboratory results and full-scale performance on Chl-a removal demonstrates the promising linear scalability of the ECO process in cyanobacteria inactivation using specific energy consumption as the key design parameter, given that the same anodes (i.e., NATO) are used.
Intracellular MC-LR was not found in the Lake Neatahwanta water samples. The treatment at 7 mA cm−2 led to an effluent extracellular MC-LR concentration (i.e., MC-LR resides in lake water) of 0.41 μg L−1 (vs. C0 = 3 μg L−1), corresponding to a destruction efficiency of 86%. Note that it is challenging to study the extracellular MC-LR degradation kinetics due to the rapid degradation in ECO within a minute. Nonetheless, the 86% destruction of MC-LR when 40% Chl-a degradation was obtained in the full-scale CMFR reactor is comparable with the results observed in the lab-scale batch reactor (>95% destruction of MC-LR when ∼40% Chl-a degradation was achieved, as shown in Fig. 3).
For all the field tests, the residual chlorine and O3 in the treated effluent are below the detection limits (both at 0.1 mg L−1). This is likely due to the short retention time of 30 seconds, which did not allow for the accumulation of residual oxidants in the continuous flow reactor. Additionally, the loss of oxidants during sample shipment is possible. Samples were transported from the boat to the shore for water analysis, and oxidant decay may have occurred during this transfer. To verify the generation of oxidants, we conducted supplementary tests at bench-scale by electrolyzing Lake Neatahwanta water at 7 and 10 mA cm−2 in batch mode (Fig. 1b). At a retention time of 30 seconds (equivalent to the hydraulic retention time of the field-scale CMFR), the O3 concentrations were measured to be 0.2 mg L−1 under 7 mA cm−2 and 0.4 mg L−1 under 10 mA cm−2. Chlorine concentrations were below the detection limit of 0.1 mg L−1 (Fig. S1b†). The O3 concentration range of 0.2–0.4 mg L−1 likely mirrors the residual O3 concentrations in the effluent immediately after exiting the reactor.
It is important to emphasize that the inactivation of algae should be achieved after a single pass through the CMFR due to the exposure of cells to locally concentrated O3 and ·OH around NATO anodes, as discussed above. The decay of 0.2–0.4 mg L−1 residual O3 during sample transportation is unlikely to contribute further to the treatment as our findings demonstrated that ozonation with O3 up to 0.8 mg L−1 did not noticeably remove Chl-a (Fig. 2d).
The current density of 7 mA cm−2 (total current of 700 A and cell voltage of 14 V in treating Lake Neatahwanta) was identified as the optimum condition, balancing the significant algae removal, low byproduct formation, and insignificant toxicity (discussed below). It is important to note that the ∼40% Chl-a removal at a short retention time of 30 s already incurred instant improvement in water clarity (Fig. S9†). Improvement in local water quality can be expected by the circulative treatment of water. The energy consumption of 0.5 Wh L−1 is also lower than the chlorine-based electrochemical HAB mitigation system (1.1 Wh L−1) reported in our previous study.43
Whole effluent toxicity tests were conducted to evaluate whether the effluent from the ECO process would negatively impact the aquatic life in the receiving water. Samples of influent (INF) and treated effluent (EFF) were collected during the operation in Lake Neatahwanta (Table S2†) and Oneida Lake (Table S3†) at 6–10 mA cm−2. In the toxicity tests, model freshwater invertebrate (Ceriodaphnia dubia) and fish (Pimephales promelas) species were exposed to INF samples, EFF samples, and lab control water (CON). Ten replicates were used for Ceriodaphnia dubia, and four replicates for Pimephales promelas for each INF, EFF, and CON exposure. Comparing the toxicity test results of samples obtained at 10 mA cm−2 (Table S2†), although Ceriodaphnia dubia survival was 100% at both 48 h (acute) and 6–7 days (chronic), reproduction is lower in EFF than INF, suggesting the treatment induced toxicity for the invertebrate species. Conversely, no effects were observed on the survival and growth of Pimephales promelas. The analysis of samples derived from operation at 6 and 7 mA cm−2 show that there were no significant survival impacts at either 48 h (acute) or 6–7 days (chronic) for either the invertebrate or fish species, with survival rates of 100%. Similarly, there were no significant effects on the reproduction of invertebrates or the growth of fish in EFF compared to INF. The whole effluent toxicity tests confirmed that the ECO process operated at 6 and 7 mA cm−2 did not induce acute or chronic toxicity in the treated effluent, echoing our conclusion that 7 mA cm−2 is the optimum current density. Lastly, it is important to note that the ECO process alone cannot remove nutrients. We are currently exploring the integration of separation processes, such as coagulation and filtration, as pretreatment steps to the ECO process. Incorporating these algae harvest processes may enable the treatment train to remove intracellular nutrients and reduce the recurrence of algal blooms.
The significant merit of the engineering of this study is the scaled-up application of the ECO process to solve real-world problems. The performance of batch mode lab-scale reactor was replicated in a boat-mounted CMFR reactor using specific energy consumption as a benchmark. In addition to the successful demonstration of HAB mitigation, we also evaluated whole effluent toxicity to show that the ECO process operated at the optimized current density of 7 mA cm−2 did not increase the effluent toxicity. This case study sets an example for the scaled-up application of electrified water treatment technology in environmental remediation.
Footnote |
† Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d4ew00490f |
This journal is © The Royal Society of Chemistry 2024 |