Yanqiu Wang
ab and
Myongsoo Lee
*b
aEngineering Research Center of Optoelectronic Functional Materials, Ministry of Education, School of Materials Science and Engineering, Changchun University of Science and Technology, Changchun, 130022, China
bDepartment of Chemistry, Fudan University, Shanghai, 200438, China. E-mail: mslee@fudan.edu.cn
First published on 28th May 2025
Supramolecular nanostructures based on the self-assembly of aromatic amphiphiles have received considerable attention because these structures based on non-covalent interactions can be dynamic, leading to switchable nanomaterials in response to external stimuli. The self-assembled materials combine the simplicity of small molecules with the versatility of self-assembly, with a wide range of applications proposed in biomedicine, nanotechnology, etc. Among the diverse self-assembled nanostructures, two-dimensional (2D) nanosheet structures are promising objects in the materials science field due to their ultrathin, large surface areas. Much research effort has been devoted to the study of 2D nanosheet structures based on diverse shapes and conformations of the aromatic segments. This review describes recent progress in the development of nanosheet structures through the self-assembly of rationally designed π-conjugated aromatic amphiphile building blocks. Potential applications, such as chiral separation and biological application, are also discussed. Various views on 2D nanosheet structures have been proposed in the literature, and in this respect, we have attempted to provide a systematic account based on our research progress in recent years. We hope that this will provide a useful reference for 2D nanosheet structures formed by the rational design of aromatic amphiphile self-assembly. We also anticipate that this strategy will provide an opportunity for broadening the application potential of 2D nanosheet structures.
Yanqiu Wang received her BS degree from Jilin Normal University in 2012 and PhD degree in chemistry from Jilin University in 2017. After completion of her PhD, she joined as an Assistant Professor at the College of Materials Science and Engineering at Changchun University of Science and Technology, Changchun, China. Her research interests are supramolecular assembly and functionalized supramolecular structures. The focus of her current research is the development of supramolecular chiral separation materials. |
Myongsoo Lee is a Professor of Chemistry at Fudan University. He received his Ph.D. degree from the Case Western Reserve University in 1992 in liquid crystal polymerization. After postdoctoral research work at the University of Illinois at Urbana, he was made a professor at Yonsei University in 1994, Seoul National University in 2009, Jilin University in 2013, and Fudan University in 2019, where he continues to work on self-assembling systems and dynamic supramolecular nanomaterials. |
Much research effort has been devoted to the study of supramolecular self-assembly structures based on various building blocks.14–19 Among diverse nanostructures, self-assembled nanosheets based on aromatic building blocks have attracted considerable attention because their interactions are robust, which makes it possible to control the size and shape of the self-assembled structures through environmental conditions.20–23 It is remarkable that an individual layered sheet structure has high potential to drive 3D stacking owing to the huge surface energy. Compared to 3D materials, 2D nanosheet structures have extremely high surface areas and many potential applications. Therefore, creating a single layered 2D nanosheet structure through supramolecular self-assembly is highly valuable. Several research groups have designed and constructed self-assembled single layered nanosheets. For example, Fernández and co-workers exploited halogen bonding (XB) as a reversible network element to regulate the photoresponsive and adaptive behavior of supramolecular 2D sheet structures.24 Liu and co-workers reported a self-assembly strategy to fabricate 2D nanosheets using imidazolium functionalized pillar[6]arene (P6) as the cationic bridging monomer and phosphotungstic acid.25,26 Tamiaki and co-workers also greatly advanced the study of self-assembled sheet structures by developing artificial chromosomal supramolecular nanosheets prepared by the self-assembly of a synthetic zinc 31-methoxy-chlorophyll derivative having amide and urea groups as the substituents at the 17-position.27 These successful examples greatly broadened the application areas of self-assembled nanosheet structures.
Typical examples of basic building blocks for self-assembly including lipid molecules,28,29 surfactants,30–33 aromatic amphiphiles,34,35 block polymers,36–38 DNA-π-conjugated amphiphiles,39–41 and peptide derivatives42–46 have been reported. Among them, amphiphile molecules, containing both hydrophobic and hydrophilic segments and the intrinsic potential of self-assembly in aqueous environments, are ideally suitable for fabricating responsive self-assembled nanostructures because of their dynamic and reversible conformational changes, which are easily triggered by different external stimuli, such as guest molecules,47–51 pH,52,53 ions,54–56 light,57,58 and temperature.59,60 In this review, we describe the recent progress in the development of 2D nanosheet structures through the self-assembly of rationally designed π-conjugated aromatic amphiphile building blocks. The nanosheet structures originate from either the lateral association of fibers or tubules, monolayer packing of aromatic amphiphiles, or transformation from scrolls. Herein, we have classified 2D nanosheet structures based on the shapes of the hydrophobic aromatic building blocks and associated them with their self-assembly behavior in aqueous solutions. Our classification will provide a better understanding of self-assembly behavior, which is key to constructing supramolecular 2D nanosheet structures. We have shown that the combination of hydrophilic chains into aromatic segments generates dynamic 2D nanosheet structures and how these self-assembled nanosheet structures exhibit switchable motion in response to external stimuli, such as temperature, guests, and pH, towards the aim of constructing smart materials. We highlight some potential applications of supramolecular nanosheet structures, such as chiral separation and biological applications.
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Fig. 1 (a) Molecular structures of cis-1 and trans-2 macrocycle isomers. (b) HPLC traces of 1 and 2 after separation. (c) Negatively stained TEM image of 1 from 0.01 wt% aqueous solution. Scale bar: 100 nm. The inset shows a Cryo-TEM image of 1 from 0.01 wt% aqueous solution. Scale bar: 200 nm. (d) Time-dependent CD spectra of 1 from 0.01 wt% aqueous solution. (e) Molecular dynamics simulation results of 1 in a water environment. Dendrimers are omitted for clarity. Time-dependent TEM images of 2 from 0.01 wt% aqueous solution after aging for (f) 30 min and (g) 3 h. Scale bars: 50 nm. The inset image shows the top view of the tubular scroll. Inset scale bar: 30 nm. (h) A representation of dynamic rolled sheets. Reproduced with permission from ref. 63. Copyright 2016 Wiley-VCH. |
However, the self-assembly of the trans-isomer 2 generates dynamic rolled sheets that are reversibly unrolled in response to a thermal signal. To investigate dynamic motion, time-dependent transmission electron microscopy (TEM) experiments were performed. Time-dependent TEM revealed that the trans-isomer 2 self-assembles into flexible nanofibers at the initial stages of self-assembly (10 min), which were arranged parallel to each other to form flat sheets after 30 min (Fig. 1f). Subsequently, the sheets spontaneously roll-up perpendicular to the fiber long axis into closely-packed scrolls with an external diameter of approximately 40 nm (Fig. 1g), and the inset image of Fig. 1g indicates that the scrolls consist of multilayer walls. CD spectroscopy measurements revealed that the signals appeared after 30 min of aging under ambient conditions and became more apparent with increasing aging time up to 3 h. These results, together with the time-dependent TEM data, suggest that the adjacent aromatic macrocycles are twisted with respect to each other in one direction, driving the planar sheets to fold perpendicular to the fiber axis into closely packed scrolls. Interestingly, the scrolls undergo reversible unfolding at 40 °C at which the oligoether chains underwent dehydration.66–69 Upon heating to 37 °C, the closely-packed scrolls gradually loosened and subsequently transformed into open sheets (Fig. 1h). In contrast to the dynamic rolled sheet structures of 2, the planar structures were maintained even at higher temperatures without any noticeable structural changes, indicating that the 2D structures were static within the temperature range of our investigations.
The above results prompted us to investigate the switchable self-assembly behavior of planar aromatic macrocycle amphiphiles. The planar aromatic macrocycle amphiphile isomers 3 and 4 were further constructed.70 The macrocycle amphiphiles self-assemble into stable, single-layered 2D sheet structures through lateral association of primary fibrils in aqueous solution (Fig. 2a). The TEM image in Fig. 2b shows the formation of flat 2D sheet structures with uniform sheet thickness (Fig. 2b). Closer examination of the images showed that the sheet consisted of longitudinal stripes with a regular spacing of ∼3.5 nm, demonstrating that the sheet assembly originated from the lateral association of preformed fibrils (Fig. 2b, inset). The formation of the planar sheet nanostructures was further confirmed using Cryo-TEM and AFM. The formation of single layered 2D sheets covered by oligoether surfaces suggests that the aromatic sheets would have thermo-responsive characteristics in aqueous solution due to the thermal dehydration behavior of the oligoether chains.65,66,71 TEM experiments were performed on the aqueous solution of cis-3 at 40 °C. In contrast to the image taken at room temperature, the image revealed thin fibrils with a uniform diameter of 4 nm (Fig. 2c), which was further confirmed by AFM measurements. This result demonstrates that the sheets can split into uniform nanofibers in response to heating. The splitting of the 2D sheets upon heating was attributed to the thermal dehydration of the dendritic ethylene oxide chains in aqueous media. Upon cooling to room temperature and then resting for 24 h, complete recovery of the original sheets was observed, indicating that the sheets underwent reversible splitting between the sheet and nanofiber states (Fig. 2a). The thermal dynamic behavior of trans-4 was similar to that of cis-3. In short, we successfully constructed thermally dynamic 2D nanosheet structures by self-assembly of geometric macrocycle amphiphile isomers.
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Fig. 2 (a) Molecular structures and schematic representation of the formation of a 2D sheet that undergoes reversible break-up into nanofibers. (b) Negatively stained TEM image of 2D sheets from 0.01 wt% aqueous solution of cis-3. The inset shows a magnified image, scale bar: 20 nm. (c) Negatively stained TEM image of cis-3 from 0.01 wt% aqueous solution at 40 °C. Reproduced with permission from ref. 70. Copyright 2021 Royal Society of Chemistry. |
Supramolecular chirality can be induced by chiral transfer from chiral moieties to self-assembled nanostructures.72 Therefore, chiral dendrimers were grafted on the side faces of tetraphenylethylene (TPE) segments which have great potential for generating 2D chiral supramolecular structures due to presence of the flat rod aromatic segment.73 Dynamic light scattering (DLS) experiments were performed on tetra-substituted molecule 5 and di-substituted molecule 6 in MeOH. The DLS data of 5 and 6 showed a diameter of ∼4 nm for both molecules, which is similar to the size of the single molecules, and fluorescence shows very weak emission, indicating that both 5 and 6 exist as molecularly dissolved states in pure MeOH, considering that TPE-based molecules are non-emissive in the dissolved state but highly enhanced emission could be seen in both the aggregated form and the solid-state.74,75 To investigate the aggregation behavior of 5 and 6, salt water (including 50 mM KF) was added to MeOH solution (0.01 wt%). With increasing water content up to 40% and with 50 mM KF salt, both the diameter and fluorescence intensity increased remarkably, indicating that addition of water to the methanol solution induced molecular assembly (Fig. 3a). The aggregation behavior of 5 and 6 at a concentration of 0.01 wt% in aqueous salt solution had been investigated using TEM and Cryo-TEM, and the images of both molecules showed 2D flat sheet structures which was further confirmed by TEM (Fig. 3b). AFM experiments were performed to obtain further information on the nanosheet structures of 5 and 6. The AFM images revealed 2D sheet structures with a thickness of ∼3.8 nm and ∼4.6 nm formed by the self-assembly of 5 and 6, respectively. Considering the thickness of 2D sheets formed by molecule 5, which is in agreement with the molecular dimension of 5 (∼3.5 nm from CPK), it is suggested that the sheet structures of 5 are based on monolayer packing. Nevertheless, the molecular dimension of 6 is ∼2.4 nm from CPK, and the observed thickness implies that the sheet structures consist of interdigitated bimolecular packing. Interestingly, when CD spectroscopy experiments were performed on the sheet structures, the sheet solution of 6 revealed a strong Cotton effect, in sharp contrast to that of 5 which is CD silent (Fig. 3c). This indicates that molecule 5 generates nonchiral 2D nanosheet structures, whereas di-substituted molecule 6 generates chiral 2D nanosheet structures. The formation mechanism of the 2D sheet structures of 5 and 6 was studied under highly diluted conditions (0.002 wt%). TEM images revealed that the solution of 5 showed small sheet-like aggregates at lower concentrations, demonstrating that the 2D sheet structures of 5 were formed by a 2D array of aromatic segments with top and bottom surfaces covered by hydrophilic dendritic segments (Fig. 3a). The diluted solution of 6 showed uniform nanofiber structures with a diameter of ∼4.6 nm together with a Cotton effect in the spectral range of the aromatic segments, which was further confirmed by AFM (Fig. 3d), demonstrating that the sheet structures of 6 originated from the lateral assembly of the helical nanofibers (Fig. 3a). The results described herein demonstrate that molecule 5 self-assembles into nonchiral 2D nanosheet structures formed by a 2D array of monomer molecules with top and bottom surfaces covered by hydrophilic dendritic segments. Molecule 6 formed a chiral sheet structure originating from the lateral assembly of helical nanofibers, suggesting that the aromatic segments were stacked on top of each other with mutual rotation.
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Fig. 3 (a) Molecular structures of tetra-substituted molecule 5 and disubstituted molecule 6, with schematic representations of their self-assembly. (b) TEM image of 6 in aqueous solution (including 50 mM KF) at 0.01 wt%, inset: Cryo-TEM image of 6 in aqueous solution (including 50 mM KF) at 0.01 wt%. (c) CD experiments of 5 and 6 in aqueous solution (including 50 mM KF) at 0.01 wt%. (d) AFM image of 6 in aqueous solution (including 50 mM KF) at 0.002 wt%. Reproduced with permission from ref. 73. Copyright 2020 Wiley-VCH. |
Although 2D nanosheet structures have been extensively studied, reports on supramolecular switching between tubules and sheets through reversible lateral association are rare. Recently, the unique self-assembly of a seesaw-shaped aromatic amphiphile structure (molecule 7) was reported (Fig. 4a), which formed unique supramolecular tubular bamboo-culm structures.76 The tubular structure possessed a hydrophobic cavity that could capture a hydrophobic guest (Fig. 4b). The addition of hydrophobic trans-azobenzene enabled the tubular culm to transform into a hierarchical sheet assembly via enhanced interactions between tubules (Fig. 4c). This transformation between tubules and sheets was also confirmed by TEM, which showed lateral connection of the tubular structures to form flat sheets. Interestingly, the transformation was reversible when UV irradiation was performed due to the conversion of trans-azobenzene to its cis-isomer. The chiral sheet structures were disassembled into their original tubular structures due to the shorter length of cis-azobenzene after 8 h of UV irradiation which decreased the lateral interactions. The full recovery of the sheet structures was achieved by resting in a dark environment, which demonstrated the reversible transformation between tubules and sheets triggered by UV irradiation due to the trans–cis isomerization of azobenzene. Such a unique switch motion between tubules and 2D nanosheet structures provides great potential for fabricating functionally reversible materials by photo-irradiation.
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Fig. 4 (a) Molecular structure of 7 and the schematic of the reversible switching between a supramolecular tubular culm and a hierarchical sheet-assembly, based on a seesaw-shaped amphiphile. (b) Negatively stained TEM image of molecule 7 (53 μM) in MeOH–H2O (40/60, v/v). The inset image shows the separated hollow-tubular interior by nodes per 1.3 nm. (c) Negatively stained TEM image and Cryo-TEM image (inset) of 7 (53 μM) with two equivalents of azobenzene in MeOH–H2O (40/60, v/v) solution. Reproduced with permission from ref. 76. Copyright 2020 Wiley-VCH. |
Synthetic supramolecules can form sophisticated nanostructures depending on their constituent molecular units. Therefore, controlled self-assembly of synthetic aromatic amphiphiles has emerged as a pivotal tool for constructing functional nanostructures. The lateral association of aromatic rods in amphiphilic molecules leading to supramolecular 2D membrane structures has been reported.77 Herein, charged rod amphiphiles 8 and 9 were synthesized, and the fluorescence of the aggregation-induced emission (AIE) luminogen 10 was modulated based on their self-assembly (Fig. 5a).78 Rod amphiphile 8 self-assembled into flat sheet structures surrounded by negatively-charged carboxylates at neutral pH (Fig. 5b). The sheets are free standing in bulk solution with a thickness of 2.8 nm, demonstrating that the aromatic rods of 8 are packed in a monolayer arrangement in which the rods are aligned parallel to the sheet plane. Then, the aggregation behavior of cationic distyrylanthracene (DSA) derivative 10 with AIE properties was investigated in the presence of self-assembled sheets of 8 in aqueous solution at pH 7.4. Upon addition of dye 10 to the negatively-charged sheets of 8, the fluorescence intensity of 10 was significantly increased, while pure 10 in pH 7.4 did not show any noticeable fluorescence emission due to the inhibition of aggregation by electrostatic repulsion between the positive charges of N,N,N-trimethylaniline groups of 10 (Fig. 5c). These results demonstrated that the negative charges on the sheet surfaces of 8 neutralized the positive charges of 10 to induce the aggregation of 10 (Fig. 5d). Amphiphile 9 with two amino groups was synthesized to confirm the electrostatic interactions between carboxylates of 8 and the ammonium groups of 10 rather than intercalation of the aromatic segments of 10 into the self-assembled rods of 8. The self-assembly of amphiphile 9 also showed a flat 2D structure in aqueous solution, which was surrounded by positively-charged ammonium groups at pH 7.4. The emission spectrum of 10 did not increase even after the addition of 9, and the NMR spectrum of 10 with the positively-charged sheet of 9 was identical to that of 10 (Fig. 5e). These results demonstrate that the aromatic segments of 10 did not intercalate into the rod segments of the sheets. Taken together, it can be considered that the fluorescence turn-on nanodomains of the positively-charged DSA dye 10 are formed by self-assembly on the negatively-charged sheet surfaces of 8 in bulk solution by electrostatic interactions.
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Fig. 5 (a) Molecular structures of 8 and 9 for self-assembled sheets with charges, and cationic distyrylanthracene derivative 10 for fluorescent synthetic rafts. (b) Negatively stained TEM image of 8 (60 mM). Reproduced with permission from ref. 77. Copyright 2018 Royal Society of Chemistry. (c) Emission spectra of different equivalents of 10 with 8 (115 mM) in 10 mM phosphate buffered saline (PBS) solutions at 421 nm excitation wavelength. (d) Representation of aggregates of 10 for the synthetic rafts on the negatively-charged sheet of 8 through electrostatic interaction. (e) 1H-NMR (500 MHz) spectra of 10 without and with sheets in D2O at 25 °C. Reproduced with permission from ref. 78. Copyright 2019 Wiley-VCH. |
As an extension of our efforts to create flat 2D sheet structures, pyrene-based aromatic amphiphiles have been synthesized, which can be triggered by environmental changes. We designed the pyrene as an optically-active aromatic segment to construct fluorescence-switching 2D materials.79 The molecule 11 consisting of an aromatic segment and oligoether dendrons grafted at opposite sides of the aromatic plane was synthesized (Fig. 6a), which formed single-layer planar sheet structures at neutral pH (pH = 7) as observed by TEM. Interestingly, the sheet structures based on pyrene units are sensitive to a small change in pH, showing strong excimer emission at pH 7, but quenching of the fluorescence emission at pH 5 without sacrificing its intact 2D self-assembled sheet structure (Fig. 6b and c). The dynamic switching behavior of the sheet structures was due to the changing packing mode of the pyrene aromatic segments. The hydrophobic and conjugated pyrene moieties showed close stacking through strong π–π interactions at pH 7, which caused the strong emission of the pyrene excimer. However, the pyridine segments were protonated at pH 5, which increased the repulsive interactions between adjacent pyridinium cations, loosening pyrene packing. We attributed the fluorescence quenching of the sheet solution to the loose packing of pyrene units (Fig. 6d). Such fluorescence emission enhancement and quenching were reversible between pH 7 and 5. We successfully constructed reversible fluorescence sheet structures by adjusting the intermolecular interactions of pyrene segments, which are promising candidates for use in fluorescence sensors.
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Fig. 6 (a) Molecular structure of amphiphile 11. (b) Fluorescence spectra of 11 (50 mM) in MeOH/H2O (1![]() ![]() ![]() ![]() |
Supramolecular self-assembly materials formed by noncovalent interactions are switchable when triggered by external stimuli such as guest molecules, pH, solvent, and temperature. With this idea in mind, π-electron rich pyrene-based aromatic amphiphiles were synthesized to facilitate charge transfer complexation with π-electron deficient 7,7,8,8-tetracyanoquinodimethane (TCNQ), so that stimuli-responsive supramolecular materials were constructed and the self-assembled nanostructures in aqueous solution were investigated (Fig. 7a).80 Pyrene amphiphile 12 was composed of a hydrophobic pyrene segment and a hydrophilic D-mannose segment, which presented micelle structures as confirmed by TEM experiments. However, after the addition of TCNQ to the aqueous solution of amphiphile 12, a color change from colorless to yellow was observed. A characteristic broad absorption band arising from intermolecular charge transfer interactions was detected at approximately 441 nm, indicating the formation of a charge transfer interaction between π-electron rich pyrene and π-electron deficient TCNQ. The maximum amount of TCNQ loading per amphiphilic molecule was 1.0 equivalent as determined by optical microscopy. The optical microscopy image of 12 complexed with 1.0 equivalent of TCNQ revealed that isolated 2D sheet structures were formed in the bulk solution (Fig. 7b). The negative staining TEM and AFM experiments revealed that the sheets were flat and uniform, consistent with the Cryo-TEM results. The small-angle X-ray diffraction analysis performed on the films of aqueous solution provided a reflection of 4.55 nm as the d-spacing between sheets (Fig. 7c), which is consistent with the thickness (4.4 nm) obtained from the AFM. The close packing of pyrene and TCNQ was confirmed by the wide-angle X-ray scattering (WAXS) pattern which showed a strong reflection at 3.4 Å associated with the π–π stacking distance. 1H-NMR spectroscopy and density functional theory (DFT) calculations further indicated parallel stacking interactions between pyrene and TCNQ. When CD measurement was performed on the charge transfer complex solution, apparent CD signals were detected in the spectral range of the aromatic segments (Fig. 7d), which indicated that the supramolecular sheet structures derived using TCNQ were chiral due to the asymmetric stacking of the aromatic segments. Interestingly, the resulting supramolecular chiral sheets showed disassembly when TCNQ was irreversibly reduced to its anion by 2.0 equivalents of sodium sulfide (Fig. 7e). The molecular orbital energy levels of pyrene, TCNQ, and the TCNQ dianion indicate that the sheet disassembly is due to the lack of charge-transfer interactions between pyrene and the TCNQ dianion. Therefore, reduction-responsive supramolecular sheets were constructed using TCNQ successfully (Fig. 7f).
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Fig. 7 (a) Molecular structures of amphiphile 12 and TCNQ. (b) Phase-contrast optical microscopy image of 12 (314 × 10−6 M) in an aqueous solution with one equivalent of TCNQ. (c) SAXS pattern of 12 with 1.0 equivalent of TCNQ after freeze-drying. (d) CD spectra of amphiphile 12 (314 μM) with different equivalents of TCNQ in an aqueous solution. (e) Negatively stained TEM image of 12 (314 × 10−6 M) with one equivalent of TCNQ sequentially treated with 2.0 equivalents of sodium sulfide in an aqueous solution. (f) Schematic representation of sheet assembly via charge-transfer interactions between 12 and TCNQ and sheet disassembly upon hydrogen-sulfide-triggered TCNQ reduction. Reproduced with permission from ref. 80. Copyright 2023 Wiley-VCH. |
Peptides, which share amino acid monomers with proteins, serve as fundamental structural frameworks in biological systems. Artificially designed β-sheet peptides have attracted considerable attention in various research areas because they are composed of biocompatible amino acids. To endow a peptide chain with the self-assembling feature, an amphiphilic peptide was synthesized by grafting the pyrene group to the peptide chain by a click reaction (Fig. 8a).81 To gain insight into the self-assembly behavior of the pyrene-based peptide 13, TEM experiments were performed under different dilution conditions. ∼3 nm sized micelle structures were obtained at 0.001 wt% and laterally-associated small fragments of flat structures were obtained at 0.003 wt% (Fig. 8b). Molecular dynamics simulations revealed that the dimer height of 13 was 3 nm, which indicated that the hydrophilic peptide helices were aligned in one direction and parallel to the flat membrane surface to form peptide bilayers with hydrophobic pyrene units (Fig. 8c). Interestingly, the flat sheet structures formed by 13 were dynamic. With an increase in the concentration of 13, the flat sheet structures transformed into vesicles, decreasing the surface energy. Therefore, 2D nanosheet structures were constructed using a pyrene-based peptide self-assembly.
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Fig. 8 (a) Chemical structure of 13. (b) TEM images of 13 at different concentrations. (c) Energy-minimized packing structure of the dimeric association of 13. Reproduced with permission from ref. 81. Copyright 2017 Royal Society of Chemistry. |
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Fig. 9 (a) Schematic representation of the capture and chiral separation of racemic phenylalanine with a chiral sheet. Reproduced with permission from ref. 73. Copyright 2020 Wiley-VCH. (b) Schematic representation of enantioselective encapsulation and further chemical transformation inside the vesicle nanoreactor. A; dendritic alkyne, D; D-enantiomer of G4, L; L-enantiomer of G4, D–A; reaction product. Reproduced with permission from ref. 81. Copyright 2017 Royal Society of Chemistry. Negatively stained TEM images of Con A on (c) synthetic rafts and (d) sheets of 9 at pH 7.4. The bottom image shows the size profiles of Con A aggregates in the range of 10 to 50 nm. Reproduced with permission from ref. 78. Copyright 2019 Wiley-VCH. (e) Specific binding of ORN 178 GFP FimH to the supramolecular sheets. TEM, SEM, and optical microscopy (phase-contrast and fluorescence) images with the supramolecular sheets. Green fluorescence is due to the green fluorescence protein (GFP) of ORN 178. The scale bar of phase-contrast optical microscopy is 20 μm. Reproduced with permission from ref. 80. Copyright 2023 Wiley-VCH. |
In particular, the dynamic and reversible nature of supramolecular interactions (such as hydrogen bonding and π–π stacking) endows nanomaterials with self-healing and stimuli-responsive capabilities. However, these properties also make nanosheets susceptible to disassembly in complex environments (such as high temperatures, extreme pH, or ionic strength), thereby limiting their long-term application under harsh conditions. Although the above research progress provides significant insights for the understanding of the formation of 2D nanosheet structures by self-assembly, more research efforts are still necessary to construct stable functional materials using aromatic amphiphiles by rational design. For example, the incorporation of dynamic covalent bonds (such as imine/disulfide bonds) and supramolecular interactions allows the construction of adaptive smart nanosheets, which exhibit both high stability and reconfigurability, enabling environmentally responsive functional switching.84–86 We anticipate that this contribution will help researchers to make further efforts to design unique aromatic amphiphiles with more functions, which will generate novel properties of supramolecular 2D nanosheet structures.
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