Engineered biosynthesis of novel spinosyns bearing altered deoxyhexose substituents

Sabine Gaisser; Christine J. Martin; Barrie Wilkinson; Rose M. Sheridan; Rachel E. Lill; Alison J. Weston; Sarah J. Ready; Clive Waldron; Gary D. Crouse; Peter F. Leadlay; James Staunton

doi:10.1039/B200536K

Engineered biosynthesis of novel spinosyns bearing altered deoxyhexose substituents†

Sabine Gaisser,*^a Christine J. Martin,^a Barrie Wilkinson,^a Rose M. Sheridan,^a Rachel E. Lill,^a Alison J. Weston,^a Sarah J. Ready,^a Clive Waldron,^b Gary D. Crouse,^b Peter F. Leadlay^ac and James Staunton^ad

Author affiliations

* Corresponding authors

^a Biotica Technology Ltd, 181A Huntingdon Road, Cambridge, UK

^b Dow AgroSciences, Indianapolis, IN, USA

^c Cambridge Centre for Molecular Recognition and Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge, UK

^d Cambridge Centre for Molecular Recognition and Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK

Abstract

Novel spinosyns have been prepared by biotransformation, using a genetically engineered strain of Saccharopolyspora erythraea, in which the β-D-forosamine moiety in glycosidic linkage to the hydroxy group at C17 is replaced by α-L-mycarose.

Chemical Communications

Engineered biosynthesis of novel spinosyns bearing altered deoxyhexose substituents†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Engineered biosynthesis of novel spinosyns bearing altered deoxyhexose substituents

Social activity

Search articles by author

Spotlight

Advertisements