13C Labeling reveals multiple amination reactions in the biosynthesis of a novel polyketide polyamine antibiotic zeamine from Dickeya zeae

Jien Wu; Hai-Bao Zhang; Jin-Ling Xu; Russell J. Cox; Thomas J. Simpson; Lian-Hui Zhang

doi:10.1039/B916307G

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

¹³CLabeling reveals multiple amination reactions in the biosynthesis of a novel polyketide polyamine antibioticzeamine from Dickeya zeae†

Jien Wu,*^a Hai-Bao Zhang,^a Jin-Ling Xu,^a Russell J. Cox,^b Thomas J. Simpson*^b and Lian-Hui Zhang^a

Author affiliations

* Corresponding authors

^a Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore 138673
E-mail: jwu@imcb.a-star.edu.sg
Fax: +65 6779 1117
Tel: +65 6586 9686

^b School of Chemistry, University of Bristol, Cantock’s Close, Bristol, UK
E-mail: tom.simpson@bristol.ac.uk
Fax: +44 (0)117 9298611
Tel: +44 (0)117 9287656

Abstract

The structure of zeamine, a novel polyamino-amide antibiotic metabolite of Dickeya zeae has been established by NMR and detailed MS analyses; labelling studies with ¹³C-labelled acetates suggest that the repeating secondary amine containing motif may be biosynthesised via a modular PKS containing aminotransferase domains.

Download options Please wait...

Supplementary files

Details of culture condition of D. zeae, isolation and spectroscopic data for zeamine 8 PDF (8376K)

Article information

DOI: https://doi.org/10.1039/B916307G
Article type: Communication
Submitted: 07 Aug 2009
Accepted: 30 Oct 2009
First published: 13 Nov 2009

Download Citation

Chem. Commun., 2010,46, 333-335

Permissions

Request permissions

¹³C Labeling reveals multiple amination reactions in the biosynthesis of a novel polyketide polyamine antibiotic zeamine from Dickeya zeae

J. Wu, H. Zhang, J. Xu, R. J. Cox, T. J. Simpson and L. Zhang, Chem. Commun., 2010, 46, 333 DOI: 10.1039/B916307G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Social activity

Fetching data from CrossRef.
This may take some time to load.

Chemical Communications