Issue 12, 2010
From the journal:

Chemical Communications

5-Carboxyfluorescein tagged N-phenylanthranilamide as a tracer reagent for fluorescence polarization: a robust method to screen MAPK pathway allosteric inhibitors†

Z. Nooshin Rezvani,a   R. John Mayera  and  Weng C. Chan*b  

* Corresponding authors

a School of Biomedical Sciences, University of Nottingham Medical School, Queen’s Medical Centre, Nottingham, UK

b School of Pharmacy, Centre for Biomolecular Sciences, University of Nottingham, University Park, Nottingham, UK
E-mail: weng.chan@nottingham.ac.uk

Abstract

Fluorescence polarization using Nα-(fluorescein-5-carbonyl)-Nε-(N-[2-fluoro-4-iodophenyl]-3,4-difluoroanthraniloyl)lysyl amide is capable of rapidly identifying inhibitor ligands that bind to an allosteric site in MEK1.

Graphical abstract: 5-Carboxyfluorescein tagged N-phenylanthranilamide as a tracer reagent for fluorescence polarization: a robust method to screen MAPK pathway allosteric inhibitors

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Article information

DOI
https://doi.org/10.1039/B925042E
Article type
Communication
Submitted
27 Nov 2009
Accepted
27 Jan 2010
First published
12 Feb 2010

Chem. Commun., 2010,46, 2043-2045

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5-Carboxyfluorescein tagged N-phenylanthranilamide as a tracer reagent for fluorescence polarization: a robust method to screen MAPK pathway allosteric inhibitors

Z. N. Rezvani, R. J. Mayer and W. C. Chan, Chem. Commun., 2010, 46, 2043 DOI: 10.1039/B925042E

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