Issue 14, 2011
From the journal:

Chemical Communications

Bioorthogonal chemical tagging of protein cholesterylation in living cells†

William P. Heal,a   Biljana Jovanovic,b   Sara Bessin,a   Megan H. Wright,ac   Anthony I. Mageebc  and  Edward W. Tate*ac  

* Corresponding authors

a Department of Chemistry, Imperial College London, Exhibition Rd., London SW7 2AZ, UK
E-mail: e.tate@imperial.ac.uk
Fax: +44 (0)20 7594 1139

b National Heart and Lung Institute, Imperial College London, Exhibition Rd., London SW7 2AZ, UK

c Institute of Chemical Biology, Imperial College London, Exhibition Rd., London SW7 2AZ, UK

Abstract

We report the first chemical probe for bioorthogonal chemical tagging of post-translationally cholesterylated proteins with an azide in living cells. This enables rapid multiplexed fluorescence detection and affinity labelling of protein cholesterylation, as exemplified by Sonic hedgehog protein, opening up new approaches for the de novo identification of cholesterylated proteins.

Graphical abstract: Bioorthogonal chemical tagging of protein cholesterylation in living cells

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Article information

DOI
https://doi.org/10.1039/C0CC04710D
Article type
Communication
Submitted
31 Oct 2010
Accepted
21 Dec 2010
First published
11 Jan 2011

Chem. Commun., 2011,47, 4081-4083

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Bioorthogonal chemical tagging of protein cholesterylation in living cells

W. P. Heal, B. Jovanovic, S. Bessin, M. H. Wright, A. I. Magee and E. W. Tate, Chem. Commun., 2011, 47, 4081 DOI: 10.1039/C0CC04710D

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