Issue 16, 2011
From the journal:

Chemical Communications

Enhancement of sortase A-mediated protein ligation by inducing a β-hairpin structure around the ligation site†

Yuichi Yamamura,a   Hidehiko Hirakawa,*a   Satoshi Yamaguchia  and  Teruyuki Nagamune*ab  

* Corresponding authors

a Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
E-mail: hirakawa@bio.t.u-tokyo.ac.jp
Fax: +81 3 5841 8657
Tel: +81 3 5841 7356

b Department of Bioengineering, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
E-mail: nagamune@bioeng.t.u-tokyo.ac.jp
Fax: +81 3 5841 8657
Tel: +81 3 5841 7328

Abstract

A Staphylococcus aureus transpeptidase, sortase A (SrtA), catalyzes selective peptide/protein ligations that have been applied to cell imaging and protein engineering, while the ligations do not proceed to completion due to their reversibility. We successfully enhanced SrtA-mediated protein ligation through the formation of a β-hairpin around the ligation site.

Graphical abstract: Enhancement of sortase A-mediated protein ligation by inducing a β-hairpin structure around the ligation site

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C0CC05334A
Article type
Communication
Submitted
02 Dec 2010
Accepted
25 Feb 2011
First published
15 Mar 2011

Chem. Commun., 2011,47, 4742-4744

Permissions

Request permissions

Enhancement of sortase A-mediated protein ligation by inducing a β-hairpin structure around the ligation site

Y. Yamamura, H. Hirakawa, S. Yamaguchi and T. Nagamune, Chem. Commun., 2011, 47, 4742 DOI: 10.1039/C0CC05334A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements