Issue 14, 2011
From the journal:

Chemical Communications

Development of a fluorescein analogue, TokyoMagenta, as a novel scaffold for fluorescence probes in red region†

Takahiro Egawa,a   Yuichiro Koide,ab   Kenjiro Hanaoka,ab   Toru Komatsu,a   Takuya Teraiab  and  Tetsuo Nagano*ab  

* Corresponding authors

a Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
E-mail: tlong@mol.f.u-tokyo.ac.jp
Fax: +81 3 5841 4855
Tel: +81 3 5841 4850

b CREST, Japan Science and Technology Agency, Sanbancho-blg, 5 Sanbancho, Chiyoda-ku, Tokyo, Japan

Abstract

We present a design strategy for fluorescence probes with a high off/on activation ratio in the red wavelength region, based on a novel fluorescein analogue in which the O atom at the 10 position of the xanthene chromophore is replaced with a Si atom. To demonstrate the usefulness of this strategy, we designed and synthesized a red-fluorescent probe for β-galactosidase, and showed that it works in live HEK293 cells.

Graphical abstract: Development of a fluorescein analogue, TokyoMagenta, as a novel scaffold for fluorescence probes in red region

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Article information

DOI
https://doi.org/10.1039/C1CC00078K
Article type
Communication
Submitted
05 Jan 2011
Accepted
11 Feb 2011
First published
28 Feb 2011

Chem. Commun., 2011,47, 4162-4164

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Development of a fluorescein analogue, TokyoMagenta, as a novel scaffold for fluorescence probes in red region

T. Egawa, Y. Koide, K. Hanaoka, T. Komatsu, T. Terai and T. Nagano, Chem. Commun., 2011, 47, 4162 DOI: 10.1039/C1CC00078K

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