Issue 26, 2011
From the journal:

Chemical Communications

A reverse transcription-free real-time PCR assay for rapid miRNAs quantification based on effects of base stacking†

Zhuoxuan Lu,a   Demin Duan,a   Rong Cao,a   Liming Zhang,a   Kexiao Zhenga  and  Jiong Li*a  

* Corresponding authors

a Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences, 398 Ruoshui Road, Dushu Lake Higher Education Town, Suzhou Industrial Park, Suzhou 215123, P. R. China
E-mail: jli2006@sinano.ac.cn
Fax: (+86) 512-62603079
Tel: (+86) 512-62872644

Abstract

A rapid and reverse transcription-free real-time PCR microRNA assay was developed based on effects of base stacking. This microRNA assay has been shown to be highly specific to homogenous miRNAs, and the procedure can be completed within 30 min starting from total RNA.

Graphical abstract: A reverse transcription-free real-time PCR assay for rapid miRNAs quantification based on effects of base stacking

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Article information

DOI
https://doi.org/10.1039/C1CC10442J
Article type
Communication
Submitted
23 Jan 2011
Accepted
04 May 2011
First published
20 May 2011

Chem. Commun., 2011,47, 7452-7454

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A reverse transcription-free real-time PCR assay for rapid miRNAs quantification based on effects of base stacking

Z. Lu, D. Duan, R. Cao, L. Zhang, K. Zheng and J. Li, Chem. Commun., 2011, 47, 7452 DOI: 10.1039/C1CC10442J

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