Issue 26, 2011
From the journal:

Chemical Communications

Engineering of a bis-chelator motif into a protein α-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy†

James D. Swarbrick,*a   Phuc Ung,a   Xun-Cheng Su,b   Ansis Maleckis,bc   Sandeep Chhabra,a   Thomas Huber,b   Gottfried Otting*b  and  Bim Graham*a  

* Corresponding authors

a Medicinal Chemistry and Drug Action, Monash Institute of Pharmaceutical Sciences, Parkville
E-mail: james.swarbrick@monash.edu, bim.graham@monash.edu
Fax: +61-3-9903 9582
Tel: +61-3-9903 9706

b Research School of Chemistry, Australian National University, Canberra
E-mail: go@rsc.anu.edu.au
Fax: +61-2-6125 0750
Tel: +61-2-6125 6507

c Latvian Institute of Organic Synthesis, Riga, Latvia

Abstract

Attachment of two nitrilotriacetic acid-based ligands to a protein α-helix in an i, i + 4 configuration produces an octadentate chelating motif that is able to bind paramagnetic lanthanide ions rigidly and with high affinity, leading to large pseudocontact shifts and residual dipolar couplings in the NMR spectrum.

Graphical abstract: Engineering of a bis-chelator motif into a protein α-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy

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Article information

DOI
https://doi.org/10.1039/C1CC11893E
Article type
Communication
Submitted
03 Apr 2011
Accepted
12 May 2011
First published
27 May 2011

Chem. Commun., 2011,47, 7368-7370

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Engineering of a bis-chelator motif into a protein α-helix for rigid lanthanide binding and paramagnetic NMR spectroscopy

J. D. Swarbrick, P. Ung, X. Su, A. Maleckis, S. Chhabra, T. Huber, G. Otting and B. Graham, Chem. Commun., 2011, 47, 7368 DOI: 10.1039/C1CC11893E

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