Issue 26, 2011
From the journal:

Chemical Communications

Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution

Yusuke Miyauchi,a   Robert Kourist,ab   Daisuke Uemuraa  and  Kenji Miyamoto*a  

* Corresponding authors

a Department of Biosciences and Informatics, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama, Kanagawa, Japan
E-mail: kmiyamoto@bio.keio.ac.jp
Fax: +81-45-566-1783
Tel: +81-45-566-1786

b Institute of Chemistry of Biogenic Resources, Technische Universität München, Schulgasse 16, D-94315 Straubing, Germany

Abstract

Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee).

Graphical abstract: Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution

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Article information

DOI
https://doi.org/10.1039/C1CC11953B
Article type
Communication
Submitted
07 Apr 2011
Accepted
26 Apr 2011
First published
11 May 2011

Chem. Commun., 2011,47, 7503-7505

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Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution

Y. Miyauchi, R. Kourist, D. Uemura and K. Miyamoto, Chem. Commun., 2011, 47, 7503 DOI: 10.1039/C1CC11953B

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