Issue 27, 2011
From the journal:

Chemical Communications

Ratiometric analysis of zidovudine (ZDV) incorporation by reverse transcriptases or polymerases via bio-orthogonal click chemistry†

Minseob Koh,a   Jongmin Park,a   Heeseon Ana  and  Seung Bum Park*ab  

* Corresponding authors

a Department of Chemistry, Seoul National University, Seoul 151-747, Korea
E-mail: sbpark@snu.ac.kr
Fax: +82 2 884 4025
Tel: +82 2 880 9090

b Department of Biophysics and Chemical Biology, Seoul National University, Seoul 151-747, Korea

Abstract

A new fluorescence-based detection method was developed to visualize zidovudine-incorporated DNA using click chemistry. This bio-orthogonal detection method was used to quantify the relative susceptibility of various DNA-synthesizing enzymes toward ZDV incorporation on the basis of ratiometric analysis of band shifts in the gel and the visual observation of cellular DNA.

Graphical abstract: Ratiometric analysis of zidovudine (ZDV) incorporation by reverse transcriptases or polymerases via bio-orthogonal click chemistry

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Article information

DOI
https://doi.org/10.1039/C1CC12518D
Article type
Communication
Submitted
29 Apr 2011
Accepted
26 May 2011
First published
10 Jun 2011

Chem. Commun., 2011,47, 7614-7616

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Ratiometric analysis of zidovudine (ZDV) incorporation by reverse transcriptases or polymerases via bio-orthogonal click chemistry

M. Koh, J. Park, H. An and S. B. Park, Chem. Commun., 2011, 47, 7614 DOI: 10.1039/C1CC12518D

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