Issue 37, 2011
From the journal:

Chemical Communications

Quantitative genotyping of single nucleotide polymorphism by single-molecule multi-color fluorescence resonance energy transfer†

Hye Ran Koh,a   Kyu Young Han,a   Jiwon Junga  and  Seong Keun Kim*a  

* Corresponding authors

a Department of Chemistry and WCU Department of Biophysics and Chemical Biology, Seoul National University, Seoul 151-747, Korea
E-mail: seongkim@snu.ac.kr
Fax: +82-2-889-5719
Tel: +82-2-880-6659

Abstract

We developed a new single nucleotide polymorphism (SNP) genotyping method based on single-molecule multi-color fluorescence resonance energy transfer (FRET). We demonstrated that this new method uses less than 1 fmol of sample and is also highly quantitative with a detection level of 1% or lower in the minor allele fraction.

Graphical abstract: Quantitative genotyping of single nucleotide polymorphism by single-molecule multi-color fluorescence resonance energy transfer

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Article information

DOI
https://doi.org/10.1039/C1CC12737C
Article type
Communication
Submitted
10 May 2011
Accepted
27 Jul 2011
First published
17 Aug 2011

Chem. Commun., 2011,47, 10362-10364

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Quantitative genotyping of single nucleotide polymorphism by single-molecule multi-color fluorescence resonance energy transfer

H. R. Koh, K. Y. Han, J. Jung and S. K. Kim, Chem. Commun., 2011, 47, 10362 DOI: 10.1039/C1CC12737C

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