Issue 31, 2011
From the journal:

Chemical Communications

Bromopyridazinedione-mediated protein and peptide bioconjugation†

Vijay Chudasama,a   Mark E. B. Smith,a   Felix F. Schumacher,a   Danai Papaioannou,b   Gabriel Waksman,b   James R. Bakera  and  Stephen Caddick*a  

* Corresponding authors

a Department of Chemistry, University College London, 20 Gordon Street, London, UK
E-mail: s.caddick@ucl.ac.uk
Tel: +44 (0)20 3108 5071

b Institute of Structural and Molecular Biology at UCL/Birkbeck, Malet Street, London, UK

Abstract

Bromopyridazinedione-mediated bioconjugation to a cysteine containing protein and a disulfide containing peptide is described. The conjugates are cleavable in an excess of thiol, including cytoplasmically-relevant concentrations of glutathione, and show a high level of hydrolytic stability. The constructs have the potential for four points of chemical attachment.

Graphical abstract: Bromopyridazinedione-mediated protein and peptide bioconjugation

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Article information

DOI
https://doi.org/10.1039/C1CC12807H
Article type
Communication
Submitted
12 May 2011
Accepted
20 Jun 2011
First published
07 Jul 2011
This article is Open Access

Chem. Commun., 2011,47, 8781-8783

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Bromopyridazinedione-mediated protein and peptide bioconjugation

V. Chudasama, M. E. B. Smith, F. F. Schumacher, D. Papaioannou, G. Waksman, J. R. Baker and S. Caddick, Chem. Commun., 2011, 47, 8781 DOI: 10.1039/C1CC12807H

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