Issue 33, 2011
From the journal:

Chemical Communications

Targeted pH-dependent fluorescent activity-based cathepsin probes†‡

Sascha Hoogendoorn,a   Kim L. Habets,b   Solène Passemard,a   Johan Kuiper,b   Gijsbert A. van der Marel,a   Bogdan I. Floreaa  and  Herman S. Overkleeft*a  

* Corresponding authors

a Leiden Institute of Chemistry, Leiden University, 2300 RA Leiden, The Netherlands
E-mail: h.s.overkleeft@chem.leidenuniv.nl
Tel: +31(0)715274342

b Dept of Biopharmaceutics LACDR, Leiden University, 2300 RA Leiden, The Netherlands

Abstract

Bifunctional, pH-activatable BODIPY dyes were developed and incorporated in mannose cluster-containing activity-based probes for cysteine proteases. Mannose receptor-dependent uptake of the probes in dendritic cells, followed by trafficking to acidic cellular compartments resulted in fluorescence as seen by live-cell imaging, and subsequent cathepsin inhibition.

Graphical abstract: Targeted pH-dependent fluorescent activity-based cathepsin probes

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Article information

DOI
https://doi.org/10.1039/C1CC12947C
Article type
Communication
Submitted
19 May 2011
Accepted
29 Jun 2011
First published
18 Jul 2011

Chem. Commun., 2011,47, 9363-9365

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Targeted pH-dependent fluorescent activity-based cathepsin probes

S. Hoogendoorn, K. L. Habets, S. Passemard, J. Kuiper, G. A. van der Marel, B. I. Florea and H. S. Overkleeft, Chem. Commun., 2011, 47, 9363 DOI: 10.1039/C1CC12947C

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