Direct radiolabelling of proteins at cysteine using [18F]-fluorosugars

Omar Boutureira; Gonçalo J. L. Bernardes; François D'Hooge; Benjamin G. Davis

doi:10.1039/C1CC13524D

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Direct radiolabelling of proteins at cysteine using [¹⁸F]-fluorosugars†

Omar Boutureira,^a Gonçalo J. L. Bernardes,^a François D'Hooge^a and Benjamin G. Davis*^a

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* Corresponding authors

^a Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford OX1 3TA, UK
E-mail: Ben.Davis@chem.ox.ac.uk
Fax: + 44 (0)1865 285002
Tel: + 44 (0) 1865 275 652

Abstract

A strategy for the site-specific attachment of 2-deoxy-2-fluorosugars to cysteine and dehydroalanine tagged proteins is reported. When combined with thionation of fluorosugars, such as the widely available ¹⁸F probe 2-deoxy-2-[¹⁸F]fluoroglucose ([¹⁸F]FDG), this methodology allows fast and direct access to site-specific [¹⁸F]FDG-labelled proteins.

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Article information

DOI: https://doi.org/10.1039/C1CC13524D
Article type: Communication
Submitted: 14 Jun 2011
Accepted: 14 Jul 2011
First published: 11 Aug 2011

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Chem. Commun., 2011,47, 10010-10012

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Direct radiolabelling of proteins at cysteine using [¹⁸F]-fluorosugars

O. Boutureira, G. J. L. Bernardes, F. D'Hooge and B. G. Davis, Chem. Commun., 2011, 47, 10010 DOI: 10.1039/C1CC13524D

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