A near-infrared fluorescent calcium probe: a new tool for intracellular multicolour Ca2+ imaging

Akihiro Matsui; Keitaro Umezawa; Yutaka Shindo; Tomohiko Fujii; Daniel Citterio; Kotaro Oka; Koji Suzuki

doi:10.1039/C1CC14045K

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A near-infrared fluorescent calcium probe: a new tool for intracellular multicolour Ca²⁺ imaging†

Akihiro Matsui,^a Keitaro Umezawa,^b Yutaka Shindo,^b Tomohiko Fujii,^b Daniel Citterio,^a Kotaro Oka*^b and Koji Suzuki*^a

Author affiliations

* Corresponding authors

^a Department of Applied Chemistry, Faculty of Science and Technology, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan
E-mail: suzuki@applc.keio.ac.jp
Fax: +81 45-566-1566

^b Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan
E-mail: oka@bio.keio.ac.jp

Abstract

We report a novel near-infrared fluorescent calcium probe (KFCA), which has good optical properties such as intense NIR fluorescence emission (670 nm, QY: 0.24), excellent ON/OFF ratio (120-fold), and good wavelength-compatibility with visible-light-emissive fluorophores (Fluo-4, DsRed2), and which is applicable for real-time dual-colour intracellular Ca²⁺ imaging.

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Article information

DOI: https://doi.org/10.1039/C1CC14045K
Article type: Communication
Submitted: 06 Jul 2011
Accepted: 27 Jul 2011
First published: 12 Aug 2011

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Chem. Commun., 2011,47, 10407-10409

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A near-infrared fluorescent calcium probe: a new tool for intracellular multicolour Ca²⁺ imaging

A. Matsui, K. Umezawa, Y. Shindo, T. Fujii, D. Citterio, K. Oka and K. Suzuki, Chem. Commun., 2011, 47, 10407 DOI: 10.1039/C1CC14045K

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Chemical Communications