Issue 19, 2011

2-Oxabutane as a substitute for internal monomer units of oligosaccharides to create lectin ligands

Abstract

The synthesis of bioactive oligosaccharides is too tedious to scale up for commercialization. However, structurally simplified glycomimetics are commercializable, if they can be synthesized much more easily than the oligosaccharides while having a comparable bioactivity. In this study, we propose a 2-oxabutane (OB) structure as an imitation of the internal monosaccharide units in oligosaccharides. Two trimannoside and three pentamannoside OB-glycomimics were synthesized in remarkably short steps. Among them, ManĪ±1-OB-2Man 10, a trimannoside mimic, showed eight-fold affinity toward concanavalin A (ConA) relative to methyl mannoside in latex agglutination lectin assay and equilibrium dialysis assay (EDA), while the other mimics showed three- to four-fold affinities. EDA indicated that the bindings between each mimic molecule and a ConA subsite were all in one-to-one stoichiometry and thus these mimics were monovalent ligands, excluding multivalence effect for the high affinities. The strong affinity of 10 could be explained by the occupation of two mannose binding sites of a ConA subsite by its two mannose units. Mimic 10 proved to be even a better ligand for ConA than the natural disaccharide ManĪ±1,2Man, while been much more easy to synthesize, thereby illustrating the potential of the approach here presented.

Graphical abstract: 2-Oxabutane as a substitute for internal monomer units of oligosaccharides to create lectin ligands

Supplementary files

Article information

Article type
Paper
Submitted
18 May 2011
Accepted
07 Jul 2011
First published
08 Jul 2011

Org. Biomol. Chem., 2011,9, 6579-6586

2-Oxabutane as a substitute for internal monomer units of oligosaccharides to create lectin ligands

L. Yang, Y. Kawada, L. Bai, D. Kubota and H. Yuasa, Org. Biomol. Chem., 2011, 9, 6579 DOI: 10.1039/C1OB05775H

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