Issue 22, 2011
From the journal:

Organic & Biomolecular Chemistry

Rational design of a cyclin A fluorescent peptide sensor†

Elena Pazos,a   Miguel Pérez,b   Hugo Gutiérrez-de-Terán,b   Mar Orzáez,c   Tatiana Guevara,c   José L. Mascareñas*a  and  M. Eugenio Vázquez*a  

* Corresponding authors

a Departamento de Química Orgánica y Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Unidad Asociada al CSIC. Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain
E-mail: joseluis.mascarenas@usc.es, eugenio.vazquez@usc.es
Fax: +34 981 595 012
Tel: +34 881 815 738

b Fundación Pública Galega de Medicina Xenómica – SERGAS, 15782 Santiago de Compostela, Spain
E-mail: hugo.teran@usc.es
Fax: +34 981 951473
Tel: +34 881 813 873

c Department of Chemical Biology, Centro de Investigación Príncipe Felipe, Valencia, Spain
E-mail: morzaez@ochoa.fib.es

Abstract

We report the design and development of a fluorescent sensor specifically designed to target cyclin A, a protein that plays a key role in the regulation of the cell cycle. Computational studies provide a molecular picture that explains the observed emission increase, suggesting that the 4-DMAP fluorophore in the peptide is protected from the bulk solvent when inserted into the hydrophobic binding groove of cyclin A.

Graphical abstract: Rational design of a cyclin A fluorescent peptide sensor

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Article information

DOI
https://doi.org/10.1039/C1OB06009K
Article type
Communication
Submitted
23 Jun 2011
Accepted
05 Aug 2011
First published
23 Aug 2011

Org. Biomol. Chem., 2011,9, 7629-7632

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Rational design of a cyclin A fluorescent peptide sensor

E. Pazos, M. Pérez, H. Gutiérrez-de-Terán, M. Orzáez, T. Guevara, J. L. Mascareñas and M. E. Vázquez, Org. Biomol. Chem., 2011, 9, 7629 DOI: 10.1039/C1OB06009K

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