Disposable electrochemiluminescent biosensor using bidentate-chelated CdTequantum dots as emitters for sensitive detection of glucose

Abstract

A novel disposable solid-state electrochemiluminescent (ECL) biosensor was fabricated by immobilizing glucose oxidase and surface-unpassivated CdTe quantum dots (QDs) on a screen-printed carbon electrode (SPCE). The surface morphology of the biosensor was characterized with scanning electron microscopy and atomic force microscopy. With dissolved O₂ as an endogenous coreactant, QDs/SPCE showed strong ECL emission in pH 9.0 HCl-Tris buffer solution with low ECL peak potential at −0.89 V. The ECL intensity was twice that with hydrogen peroxide as coreactant at the same concentration. This phenomenon meant the ECL decreased upon consumption of dissolved O₂ and thus could be applied to the construction of oxidase-based ECL biosensors. With glucose oxidase as a model enzyme, the biosensor showed rapid response to glucose with a linear range of 0.8 to 100 μM and a detection limit of 0.3 μM. Further detection of glucose contained in human serum samples showed acceptable sensitivity and selectivity. This work provided a promising application of QDs in ECL-based disposable biosensors.