Issue 6, 2012

Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices

Abstract

We introduce a label-free approach for sensing polymerase reactions on deoxyribonucleic acid (DNA) using a chelator-modified silicon-on-insulator field-effect transistor (SOI-FET) that exhibits selective and reversible electrical response to pyrophosphate anions. The chemical modification of the sensor surface was designed to include rolling-circle amplification (RCA) DNA colonies for locally enhanced pyrophosphate (PPi) signal generation and sensors with immobilized chelators for capture and surface-sensitive detection of diffusible reaction by-products. While detecting arrays of enzymatic base incorporation reactions is typically accomplished using optical fluorescence or chemiluminescence techniques, our results suggest that it is possible to develop scalable and portable PPi-specific sensors and platforms for broad biomedical applications such as DNA sequencing and microbe detection using surface-sensitive electrical readout techniques.

Graphical abstract: Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices

Supplementary files

Article information

Article type
Paper
Submitted
06 Oct 2011
Accepted
30 Dec 2011
First published
19 Jan 2012

Analyst, 2012,137, 1351-1362

Label-free electrical detection of pyrophosphate generated from DNA polymerase reactions on field-effect devices

G. M. Credo, X. Su, K. Wu, O. H. Elibol, D. J. Liu, B. Reddy, T. Tsai, B. R. Dorvel, J. S. Daniels, R. Bashir and M. Varma, Analyst, 2012, 137, 1351 DOI: 10.1039/C2AN15930A

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